摘要
为了提升棉花和花生产量,开展棉花-花生轮作模式研究,分析轮作模式下土壤根系微生物的群落结构与功能。以棉花连作、花生连作、棉花花生轮作、花生棉花轮作和休耕为研究对象,探明轮作后作物根际土壤微生物群落结构变化,利用16S rRNA基因高通量测序技术分析根系细菌群落结构与功能。结果发现:轮作提高了根系细菌群落的多样性;土壤样本中共获得5009952个有效序列,休耕模式下OTU(operational taxonomic units)含量最多。在轮作种植模式中变形菌门、酸杆菌门和放线菌门为优势菌门;其次轮作模式增加了厚壁菌门、拟杆菌门和芽单胞菌门的丰富度。根据功能不同,将菌群共划分为8类,轮作出现较多微生物基因遗传相关的功能类群,整体以代谢相关的类群居多,与人类和植物病原菌的功能蛋白组富集明显。由此得出,棉花,花生轮作能够提高微生物丰富度,增加多样性,改变土壤微生物群落结构,促进作物根系对营养物质的吸收,提高作物产量。
To enhance the yields of cotton and peanut,bacterial community structure and function in root soil under cotton-peanut rotation were studied.Five treatments were investigated,as continuous cotton cultivation,continuous peanut cultivation,cotton-peanut rotation,peanut-cotton rotation,and fallow land.High-throughput gene sequencing of 16S rRNA was used to analyze bacterial community structure and function.Results revealed that crop rotation increased the diversity of root-associated bacterial communities.A total of 5009952 valid sequences were obtained from soil samples,with the highest number of OTUs(operational taxonomic units)under fallow land treatment.In rotational cropping systems,Proteobacteria,Acidobacteria,and Actinobacteria were identified as dominant phyla.Additionally,richness of Firmicutes,Bacteroidetes,and Gemmatimonadetes were increased under rotational patterns.Based on different functions,the microbial groups were categorized into 8 classes.Under rotations modes,higher prevalence of functional groups related to microbial genetic inheritance was found.Metabolic-related groups were predominant overall,and significant enrichment of functional protein sets were found associated with human and plant pathogens.These results indicated that cotton-peanut rotation could increase microbial richness and diversity,alter soil microbial community structures,promote nutrient absorption by crop roots,and ultimately enhance crop yields.
作者
崔福洋
侯献飞
苗昊翠
贾东海
顾元国
陈晓露
李强
施俊杰
汪天玲
高君
胡凤仪
宋贤明
张云
罗玉涛
黄奕
尚随腾
张帆
CUI Fu-yang;HOU Xian-fei;MIAO Hao-cui;JIA Dong-hai;GU Yuan-guo;CHEN Xiao-lu;LI Qiang;SHI Jun-jie;WANG Tian-ling;GAO Jun;HU Feng-yi;SONG Xian-ming;ZHANG Yun;LUO Yu-tao;HUANG Yi;SHANG Sui-teng;ZHANG Fan(Yili Normal University,Yili 835000,China;Institute of Cash Crops,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China)
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2024年第3期625-634,共10页
Chinese Journal of Oil Crop Sciences
基金
国家重点研发计划(2022YFD1000105)
自治区“天山英才”培养计划-现代农牧业发展高层次人才(2023SNGGGCC018)
国家花生产业技术体系(CARS-13)
新疆维吾尔自治区油料产业技术体系(XJARS-05)。
