摘要
文章旨在分析纳木错裸鲤(Gymnocypris namensis)肠道微生物的群落结构和多样性特征,并对其潜在功能进行探索,从而为其健康稳定生长和发育以及保持纳木错生态系统的稳定性提供数据支撑。文章通过Illumina Miseq平台对纳木错裸鲤肠道微生物进行高通量测序,共计得到优化后的序列554607条,优化总碱基数229224641 bp。经DADA2降噪后,8个样本共产生346272条序列,获得2598个ASVs,统计共得到26个门、63个纲、134个目、189个科、251个属、361个种。雌、雄纳木错裸鲤在f__Desulfosarcinaceae、f__Rubritaleaceae、f__norank_o__norank_c__Berkelbacteria这三个菌属之间存在显著差异(P<0.05)。功能预测结果显示,肠道微生物编码的基因与蛋白质加工、合成以及新陈代谢紧密相关,涉及翻译,核糖体结构和生物发生及氨基酸运输和代谢功能类群的相对丰度较高。
The purpose of this paper was to analyze the community structure and diversity characteristics of the intestinal microorganisms in Gymnocypris namensis,and to investigate the possible roles of the intestinal microorganisms,so as to provide data supports for the healthy and stable growth,development and stability of Gymnocypris namensis,and the stability of the Namtso ecosystem.This paper used the Illumina Miseq platform for high-throughput sequencing of intestinal microorganisms in Gymnocypris namensis,the total number of optimized sequences was 554607,and the total number of optimized bases was 229224641bp.After DADA2 denoising,a total of 346272 sequences were generated from 8 samples,and 2598 ASVs were obtained.A total of 26 phyla,63 classes,134 orders,189 families,251 genera and 361 species were identified.There is significant difference between female and male Desulfosarcinaceae in f__Rubritaleaceae and f__norank_o__norank_c__Berkelbacteria(P<0.05).Functional prediction results show that the genes encoded by intestinal microorganisms were mostly related to protein processing,synthesis and metabolism,and the relative abundance of translation,ribosome structure and biogenesis,as well as amino acid transport and metabolic functional groups was relatively high.
作者
席建宁
刘丛丛
王艳梅
周永洪
XI Jianning;LIU Congcong;WANG Yanmei;ZHOU Yonghong(School of Ecology and Environment,Tibet University,Lhasa 850000,China;Tibet Museum of Natural Science,Lhasa 850000,China)
出处
《工业微生物》
CAS
2024年第3期169-172,共4页
Industrial Microbiology
基金
西藏自治区重点研发项目“西藏杀虫真菌资源的发掘及在设施农业中的应用”(编号:XZ202001ZY0036N)。
关键词
纳木错裸鲤
肠道微生物
多样性
功能预测
Gymnocypris namensis
intestinal microorganisms
diversity
fuction prediction
