摘要
为了促进堆肥中木质纤维素降解并了解其中微生物群落代谢功能多样性,该文主要采用Biolog微平板法,考察了添加菌剂对牛粪堆肥中木质纤维素降解及微生物群落代谢能力的影响。结果表明:堆肥结束时,菌剂处理的堆肥中纤维素、半纤维素和木质素的降解率分别比对照提高了31.31%、19.57%和14.33%;对平均色度变化分析表明接种菌剂增加了堆肥高温期微生物的细胞代谢能力;Shannon指数、Simpson指数和McIntosh指数分析均显示添加菌剂可以显著提高堆肥前期的微生物多样性;主成分分析结果显示,添加菌剂提高了堆体中微生物对双亲化合物、聚合物、氨基酸和氨基化合物等碳源的代谢能力,从而可促使有机质被更高效降解。
The effects of inocula on lignocellulose degradation and microbial communities metabolic profiles during cattle manure compost process are studied using the Biolog method mainly in order to promote lignocellulose degradation and understand the microbial community diversity of metabolic functions. The results show that the degradation rates of cellulose,hemicellulose and lignin in the in-oculated compost increas by 31 . 31%,19 . 57% and 14 . 33% respectively compared with the control＆amp;nbsp;at the end of compost. The analysis on average well color development shows that the inocula can increase the metabolic rate of cells at thermophilic stage of the compost. Analysis for Shannon index, Simpson index and McIntosh index shows that the addition of the inocula can increase the microbial diversity significantly in the early stage of the compost. The results of principal component analysis indicate that the metabolic capability on amphiphile, polymers, amino acids and amides carbon substrates of microorganisms in compost is enhanced by the inocula,which results in more efficient degradation of organic substance.
出处
《南京理工大学学报》
EI
CAS
CSCD
北大核心
2014年第1期181-186,共6页
Journal of Nanjing University of Science and Technology
基金
国家自然科学基金(31272484
31372351)
省部级基金(1252CG2H26)
黑龙江省教育厅基金(12531021)
东北农业大学博士启动基金(2012RCB34)
