摘要
采用室内小型堆肥反应器堆肥，测定CMC酶活性和β-葡萄糖苷水解酶活性，测定堆肥物料中纤维素、半纤维素和木质素相对含量，利用高效液相法测定葡萄糖和纤维二糖。结果表明，堆肥过程中，堆体温度前3 d迅速上升至45℃，进入高温期，维持8 d后进入降温期，最后与环境温度变化一致；β-葡萄糖苷水解酶活性第4天达最大值（1.482μmol p-Nitr·g-1DW·min-1），并在降温腐熟期维持在0.429～0.533μmol p-Nitr·g-1 DW·min-1范围内；CMC酶活性变化趋势与β-葡萄糖苷水解酶活性相似，并在第7天达最大值（47.67μg glucose·g-1 DW·min-1）；纤维素和半纤维素降解趋势相似，均在高温期降解明显；木质素相对含量总体变化较小，且在降温腐熟期小幅增加。结果显示，CMC酶活性与纤维素和半纤维素降解呈正相关。
After the composting in the self-made composting bioreactor, the enzyme dynamics of CMCase andβ-glucosidase determined and the content of cel ulose, hemicel ulose and lignin in the compost pile were measured, in addition the content of glucose and cel obiose were determined using HPLC. The results showed that the temperature of the pile up to 45℃in the first 3 days of composting, the thermophilic phase started, the thermophilic phase lasted for 8 days. After the peaking temperature, the temperature of pile was approached to the background in the cooling phase. The activity of β-glucosidase reached the peak（1.482 μmol p-Nitr·g-1 DW·min-1） on the 4th day and stabilized range from 0.429 to 0.533 μmol p-Nitr·g-1DW·min-1. The activity trend of CMCase was similar toβ-glucosidase, reached the peak of 47.67μg glucose·g-1DW·min-1 on the 7th day. The amounts of cel ulose and hemicel ulose were reduced during the thermophilic phase while the amount of lignin was increased slightly in the cooling phase. The results revealed that the degradation of the cel ulose and hemicel ulose was a positive correlation to the enzyme dynamics of CMCase.
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2016年第6期33-40,共8页
Journal of Northeast Agricultural University
基金
国家自然科学基金项目(31300428)
黑龙江省自然科学基金项目(C2015005)
黑龙江省教育厅项目(12541009)
黑龙江省博士后科研启动基金项目(LBH-Q13017)
