摘要
【目的】研究链霉菌（Streptomyces thermovulgaris）在农业废弃物资源化利用中的作用及其生物学机理。【方法】以含水量为60％～65％的小麦秸秆段为原料，按菌料质量比1：120接种链霉菌菌剂，以不接种链霉菌组为对照，采用稀释涂布平板法、BIOLOG法和分光光度法研究链霉菌对小麦秸秆堆肥体系中微生物群落结构及酶活性的影响。【结果】在整个堆肥过程中，接种（＋M）和不接种（-M）处理的细菌和放线菌数量均呈现高-低-高的变化趋势，真菌数量呈降低的趋势。接种链霉菌菌剂，改变了堆体中细菌群落结构组成，堆体细菌总代谢活性（AWCD）和细菌群落多样性均增加。主成分分析结果显示，在整个堆肥过程，不接菌处理堆体细菌群落结构典型变量值的变异很小，而接菌处理的变异较大。整个堆肥过程，接菌处理的蔗糖酶活性比不接菌处理高50．07％，蛋白酶活性高21．63％。【结论】接种链霉菌菌剂能够提高堆体细菌数量，提高堆体蔗糖酶和蛋白酶活性，但接种处理堆体中细菌群落稳定性低于不接菌处理。
[Objective] This study was to assess the role and biology mechanism of Streptornyces thermovulgaris agent in agriacultural waste utilization. [Method] The experiment wag conducted by wheat straw with 60%--65% water content,and quality materials by inoculum inoculated with 1 ： 120. The non-inoculated treatment was set as control. Meanwhile, microbial cultural, BIOLOG and spectrophotometry method were employed to investagate the effects of S. thermovulgaris inoculation on microbial community and enzyme activities during wheat straw composting process. [Result] The trends of the number of bacteria and antionmies were high-low-high,and the number of fungi decreased during the composting process. Bacteria community structure was altered after S. thermovulgaris agent inoculation. AWCD and numbers of compunities increased clearly. Principal component analyses indicated that the variation of bacteria community diversity of inoculation treatment was larger than that of non-inoculation treatment throughout the composting process. The average ucrose enzyme activity and protein enzyme activity of inoculation treatment were higher than those of non-inoculation treatment by 50.07% and 21.63% respectively. [Conclusion] The number of bacteria, sucrose enzyme activity and protein enzyme activity were promoted after S. therrnovulgaris agent inoculation, however, the stability of bacteria community in inoculation treatment was lower than that of non-inoculation treatment.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2009年第9期134-138,共5页
Journal of Northwest A&F University(Natural Science Edition)
基金
国家自然科学基金项目(50579066)
关键词
链霉菌
小麦秸秆
堆肥
微生物群落
酶活性
Streptomyces thermovulgaris
wheat straw
composting
microbial community
enzyme activity
