摘要
目的建立生物信号采集系统结合人工肉眼辅助观察评价油茶皂素的镇咳作用的量效关系。方法采用生物信号采集系统,利用呼吸波振幅变化率结合人工肉眼辅助观察,评价油茶皂素剂量变化对豚鼠咳嗽次数、咳嗽潜伏期的影响,并经多元线性回归得出油茶皂素镇咳量效关系方程。结果与正常呼吸波比,首次咳嗽波平均振幅变化率为2335%,末次为125%;油茶皂素75、100、175、200 mg/kg等剂量均可明显减少豚鼠咳嗽次数(P<0.05),回归方程为y=54.4576-0.2693x+0.0011x^(2);75,100、125、175、225 mg/kg等剂量均能明显延长豚鼠咳嗽潜伏期(P<0.05),回归方程为y=27.1679-0.4169x+0.0014x^(2)。结论生物信号采集系统结合人工肉眼辅助观察能客观评价油茶皂素镇咳量效关系,实验表明油茶皂素用药剂量与镇咳效应之间呈非线性关系。
Objective To establish a biological signal collection system combined with artificial visual observation to evaluate the dose-response relationship of the cough suppressive effect of Camellia oleifera saponins.Methods A biological signal acquisition system was used to evaluate the effect of changes in the dosage of Camellia oleifera saponin on the number of coughs and cough latency in guinea pigs,using respiratory wave amplitude change rate combined with artificial visual observation.The dose-response relationship equation of Camellia oleifera saponin for cough suppression was obtained through multiple linear regression.Results Compared with normal respiratory waves,the average amplitude change rate of the first cough wave was 2335%,and the last one was 125%.The same dose of Camellia oleifera saponin 75、100、175、200 mg/kg can significantly reduce the number of coughs in guinea pigs(P<0.05),and the regression equation is y=54.4576-0.2693x+0.0011x^(2).The same dose of 75、100、125、175、225 mg/kg can significantly prolong the cough latency in guinea pigs(P<0.05),with a regression equation of y=27.1679-0.4169x+0.0014x^(2).Conclusion The combination of biological signal acquisition system and artificial visual observation can objectively evaluate the dose-response relationship of Camellia oleifera saponin in relieving cough.The experiment shows that there is a non-linear relationship between the dosage of Camellia oleifera saponin and its antitussive effect.
作者
李祚勇
唐远鹏
陈芳辉
曾韬慧
林金海
LI Zuoyong;TANG Yuanpeng;CHEN Fanghui;ZENG Taohui;LIN Jinhai(The First Affiliated Hospital of Gannan Medical University,Ganzhou,Jiangxi 341000)
出处
《江西医药》
CAS
2024年第3期261-263,267,共4页
Jiangxi Medical Journal
基金
赣州市科技局课题,编号GZ2023ZSF165。
关键词
油茶皂素
镇咳作用
生物信号采集系统
量效关系
Camellia oleifera saponins
Anticough effect
Biological signal acquisition system
Quantity effect relationship
