摘要
目的:筛选逍遥散抗抑郁作用的最佳剂量,为提高临床疗效奠定基础。方法:采用慢性轻度不可预知应激模型(CUMS),通过2次实验,造模的同时给予不同剂量的逍遥散加以治疗,实验Ⅰ使用阿米替林作为阳性对照,实验Ⅱ使用文拉法辛和百忧解作为阳性对照。观察大鼠行为学变化,测定大鼠体重、旷场和糖水消耗实验等行为学指标;并收集尿液生物样本,采用GC-MS分析,数据用SPSS和SIMCA-P软件进行分析。结果:通过2次实验的行为学,显示对照组和模型组具有显著性差异,同时GC-MS数据分析,2组几乎没有交叉,能够很好的分开,均表明造模成功;各给药组与阳性药组和空白组相比,发现给药剂量为46.3 g·kg-1生药量时与对照组和文拉法辛阳性药组更加接近,比阿米替林和百忧解阳性药组抗抑郁效果好。结论:逍遥散具有明显的抗抑郁作用,且经过2次实验均证明46.3 g·kg-1为大鼠最佳剂量,提示临床上人的日用剂量(原处方生药材比例)约为370 g。
Objective：To screen the optimal dose of Xiaoyaosan in rats and to improve the anti-depression clinical effect.Method： In this research,two experiments were processed using the model of chronic unpredictable mild stress（CUMS）.Amitriptyline and fluoxetine hydrochloride,venlafaxine hydrochloride were used as positive control,respectively.Behavioral changes,such as the weight,open-field,and sugar consumption,were measured.The urine was collected and analyzed by GC-MS,and the result was analyzed with SPSS and SIMCA-P.Result： The results of behavior changes and GC-MS suggested that the control group and model group were significantly different,which indicated that the model was successful.Comparing the treated group with the positive control group and the control group,the dose of 46.3 g·kg^-1 was closest to the control group,there was no significant difference.Conclusion： Xiaoyaosan has the obvious anti-depression effect,and 46.3 g·kg-1 was proved the optimal dose.
出处
《中国实验方剂学杂志》
CAS
北大核心
2010年第13期194-198,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
科技部国际合作项目(2008DFA30430)
国家自然科学基金项目(30772759)
山西省科技厅/国际科技合作项目(2008081043)
山西省人事厅/人才引进与开发专项资金
