摘要
该研究采用液态发酵法发酵鸡腿菇,鸡腿菇纤溶酶活力为评价指标,通过单因素试验、Plackett-Burman试验及Box-Behnken试验对其培养基组成和培养条件进行优化。结果表明,最佳培养基的组成为果糖2.9%,豆饼粉3.6%,KH2PO_(4)0.25%,MgSO_(4)0.2%。最佳培养条件为装液量50 mL/250 mL,接种量10%,发酵温度24℃,发酵时间6 d,摇床转速160 r/min。在此优化培养基及培养条件下,纤溶酶活力为136.89 U/mL,比优化前提高了4.28倍。
Coprinus comatus was fermented with liquid fermentation method,using fibrinolytic enzyme activity as the evaluation index,the medium composition and culture conditions were optimized by single factor experiments,Plackett-Burman experiments and Box-Behnken experiments.The results showed that the optimal medium composition was fructose 2.9%,soybean cake 3.6%,KH2PO_(4)0.25%,MgSO_(4)0.2%.The optimal culture conditions were liquid volume 50 ml/250 ml,inoculum 10%,fermentation temperature 24℃,time 6 d,and shaking speed 160 r/min.Under the optimized medium and culture conditions,the fibrinolytic enzyme activity was 136.89 U/ml,which was 4.28 times higher than that before optimization.
作者
王今雨
刘晓兰
景言
郑喜群
WANG Jinyu;LIU Xiaolan;JING Yan;ZHENG Xiqun(College of Food Engineering,Harbin University of Commerce,Harbin 150028,China;Key Laboratory of Corn Deep ProcessingTheory and Technology of Heilongjiang Province,College of Food and Bioengineering,Qiqihar University,Qiqihar 161006,China;College of Food,Heilongjiang Bayi Agricultural University,Daqing 163319,China)
出处
《中国酿造》
CAS
北大核心
2023年第11期189-196,共8页
China Brewing
基金
黑龙江省重点研发计划指导类项目(GZ20210097)
国家自然科学基金面上项目(31171744)
黑龙江省自然科学基金重点项目(ZD201305)
黑龙江省省属本科高校基本科研业务费项目(145209314)。
关键词
鸡腿菇
纤溶酶
液体发酵
培养基
培养条件
响应面优化
Coprinus comatus
fibrinolytic enzyme
liquid fermentation
medium
culture condition
response surface optimization
