摘要
目的测定分离纯化后的芜菁多糖均一组分BP1和BP2含量及纯度,并对其进行结构分析。方法经DEAE-纤维素52和sephadex-G150柱层析分离纯化BP1和BP2,采用蒽酮-硫酸法在紫外-可见分光光度计进行纯度测定;采用比旋光度法、气相色谱法、傅里叶红外光谱法、核磁共振法等方法进行结构分析。结果BP1和BP2在分离得到的7种多糖组分中含量较高,纯度测定结果中在260 nm和280 nm处未见到蛋白质和核酸的吸收峰。BP1组分相对分子质量为110662,[α]为+18.83;BP2组分相对分子量为37844,[α]为+12.00。BP1、BP2均由阿拉伯糖(Ara)、甘露糖(Man)、葡萄糖(Glc)和半乳糖(Gal)组成,其摩尔比分别为为1∶2.90∶16.48∶1.52和1.24∶2.03∶15.24∶1。BP1主链由α-D-Man-(1→,→6)α-D-Glc-(1→跟→2,6和1→连接的β-D-Gal组成,α-D-Man-(1→,→6)α-D-Glc-(1→组成支链;BP2主链由→6)α-D-Ara-(1→,→6)α-D-Man-(1→和→6,1→)连接的β-D-Glc组成,→6和1→)连接的β-D-Glc组成支链。结论本研究初步成功推测出多糖均一组分BP1、BP2的结构特征并给后期芜菁多糖的结构鉴定提供了可靠的理论基础。
Objective To determine the content and purity of uniform content BP1 and BP2 polysaccharides from Brassica rapa L.,and to analyze the structures.Methods BP1 and BP2 wereseparated and purified by DEAE-cellulose 52 and Sephadex-G150 column chromatography,and thepurity were determined by Uv-Visible spectrophotometry and anthrone-sulfuric acid method.The molecular weights andthe specific rotation[α]were determined,and the structures were analyzed byGC,IR and NMR.Results The contents of BP1 and BP2 in the 7 components of polysaccharide were highestand did not show obvious absorption peaksin protein and nucleic acid at 260 nm and 280 nm after sephadex-g150isolated.Therelative molecular mass of component BP1 was 110662,the specific rotation was+18.83;The relative molecular massof component BP2 was 37844,and the specific rotation was+12.00.BP1 and BP2 were composed of Arabinose,Mannose,glucose and Galactose;the molar ratios were 1:2.90:16.48:1.52 and 1.24:2.03:15.24:1.Component BP1 backbone consistedα-D-Man-(1→,→6)α-D-Glcp-(1→and→2,6)β-D-Galp-(1→,α-D-)Man-(1→,→6)α-D-GlCp-(1→formsa branching chain;component BP2 backbone consists of→6)(α-D-Araf→,→6)α-D-Man-(1→and→6)β-D-Glcp-(1→,the branched chainconsists of→6)β-D-Glcp-(1→.)Conclusion In this study,the structural characteristics of Brassica rapa L.components BP1 and BP2 were predicted and reliable theoretical basis was provided for the later structural identification of Brassica rapa L.polysaccharides.
作者
米合热尼沙·阿木热江
卡地尔亚·库尔班
古娜娜·对山别克
海力茜·陶尔大洪
Miherenisha Amurejiang;Kadierya Kuerban;Gunana Duishanbieke;Hailiqian Taoerdahong(College of Pharmacy,Xinjiang Medical University,Urumqi 830017,China;Pharmaceutical Department,Affiliated Tumor Hospital of Xinjiang Medical University,Urumqi 830011,China)
出处
《新疆医科大学学报》
CAS
2022年第11期1336-1340,共5页
Journal of Xinjiang Medical University
基金
国家自然科学基金(81960765)。
关键词
芜菁
均一多糖
分离纯化
结构分析
Brassica rapa L.
uniform polysaccharides
separated and purified
structural analysis
