摘要
类器官模型在发育生理学研究、疾病模型构建、药物筛选中发挥着日趋重要的作用。然而传统的类器官培养方式操作繁复、标准化程度低、无法复现体内生理/病理微环境, 导致获得的类器官产量低、均一性差、成熟度低, 难以满足研究及应用需求。作为一种精确操控微量流体的新兴技术, 微流控芯片既可对类器官培养的微环境因素(如生化因子浓度、流体剪切力、营养供应等)进行精确控制, 也能够与微加工技术、传感技术等集成以构建器官特异性微环境并实现对类器官的连续监测, 有望替代传统培养方式, 使类器官在基础研究和生物医学应用中发挥更大的作用。本综述系统介绍了微流控芯片技术在不同来源的类器官的研究中的应用, 并对其局限性和发展前景进行了探讨。
Organoids are playing a significant role in developmental biology,disease modeling and drug screening.However,conventional organoid technologies are complex in manipulations and meet challenges for standardization,which greatly limit their applications.Furthermore,these methods can not precisely recapitulate the real physiological/pathological microenvironment in vivo,resulting in organoids with poor homogeneity and low maturity.All the facts make the current organoid technology insufficient to meet the increasing demand of clinical research.Microfluidics is an emerging technology that enables precise fluid manipulation at micro-scale.It has the ability to accurately control the microenvironment factors(such as biochemical factor gradients,shear force,nutrient supply and so on)during organoid culture.In addition,the construction of microfluidic chips is highly flexible and can integrate with microsystems such as biosensors to simulate different kinds of organs or enable continuous monitoring.Therefore,microfluidics has the potential to replace traditional methods and endow organoids with great significance in fundamental research and biomedical applications.This review systematically introduces the role of microfluidics in different kinds of organoid technologies as well as discusses its limitations and prospects.
作者
陈序
蔡博
刘文宇
代佳豪
王国斌
王琳
王征
Chen Xu;Cai Bo;Liu Wenyu;Dai Jiahao;Wang Guobin;Wang Lin;Wang Zheng(Research Center for Tissue Engineering and Regenerative Medicine,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China;Department of Gastrointestinal Surgery,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China;Department of Clinical Laboratory,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China)
出处
《中华实验外科杂志》
CAS
北大核心
2022年第6期1215-1221,共7页
Chinese Journal of Experimental Surgery
基金
湖北省技术创新专项(重大项目)(2018ACA136)。
