摘要
[目的]研究桑树中HD-Zip Ⅰ亚家族成员的进化及结构等特点,明确该家族基因的器官特异性表达模式,阐明ABA及淹水、盐和脱水处理对桑树HD-Zip Ⅰ基因表达水平的影响。[方法]通过桑树基因组数据库鉴定桑树HD-Zip Ⅰ基因,并进行生物信息学分析;利用桑树与拟南芥HD-Zip Ⅰ蛋白的序列比对结果,构建系统进化树;利用桑树RNA-seq数据分析桑树HD-Zip Ⅰ基因的组织/器官特异性表达谱;利用qRT-PCR分析桑树HD-Zip Ⅰ基因在激素及非生物胁迫处理下的表达模式。[结果]共鉴定出14个桑树HD-Zip Ⅰ基因,根据进化关系可进一步将其分为6个分枝,各分枝内的成员具有相似的基因结构及保守基序。RNA-seq数据分析发现,MnHD-Zip 2和MnHD-Zip 6在根、枝条、冬芽、雄花和叶片中均呈现高水平表达。激素处理后的基因表达分析发现,MnHD-Zip 8、MnHD-Zip 9、Mn HD-Zip 11、MnHD-Zip 12和MnHD-Zip 13受到ABA不同程度的上调诱导,桑树其余HD-Zip Ⅰ基因的表达水平均受到ABA不同程度的抑制。非生物胁迫处理表达分析发现,桑树HD-Zip Ⅰ亚家族基因均受到3种非生物胁迫不同程度的诱导表达。[结论]桑树β分枝成员受到盐和脱水胁迫的显著诱导表达,且在各种器官中也有较广泛的高表达,因此,推测这些基因在桑树生长发育及逆境胁迫中均发挥着重要的调控作用。此外,MnHD-Zip 8和MnHD-Zip 12受到了盐、淹水及脱水胁迫的显著诱导,由此推测它们在桑树逆境胁迫响应中具有潜在重要功能。
[Objective] The aims of this study were to investigate the gene structure and phylogenetic of the mulberry(Morus alba L.) HD-Zip Ⅰ subfamily members, clarify the tissue-specific expression of these genes in different organs, and reveal the expression level of the family genes under ABA and abiotic stress.[Method] Identification of putative HD-Zip Ⅰ subfamily genes from MorusDB and bioinformatics analysis were performed. The evolutionary tree was constructed based on the multiple sequence alignment of the Arabidopsis and mulberry. RNA-seq data was used to analyze the tissue-specific expression of mulberry HD-Zip Ⅰ genes. The transcription level of the family genes under hormone and abiotic stress was detected by qRT-PCR. [Result] A total of 14 HD-Zip Ⅰ genes were identified in the mulberry genome. These members could be divided into six classes by phylogenetic tree. Members in the same class were similar in the gene structure and protein motifs. The expression level of both MnHD-Zip 2 and MnHD-Zip 6 were high in root, branch, winter bud, male flower, and leaf according to the RNA-seq data. All the Mn HD-Zip Ⅰ gene except MnHD-Zip 8, MnHD-Zip 9, MnHD-Zip 11, MnHD-Zip 12, and MnHD-Zip 13 were inhibited by ABA. The result of qRT-PCR showed that all the mulberry HD-Zip Ⅰ genes could be induced by the abiotic stresses.[Conclusion] The β clades genes that were highly expressed in most of the organs were observably induced by the NaCl and dehydration. These results indicates that genes from the β clades of MnHD-Zip Ⅰ play important roles in the development and stress response. In addition, MnHD-Zip 8 and MnHD-Zip 12 were dramatically up regulated by waterlogging, NaCl and dehydration, which indicates that these genes have essential roles in stress regulation of mulberry.
作者
胡景涛
李彦杰
段艳艳
阮宇
顾欣
肖国生
HU Jing-tao;LI Ya-njie;DUAN Yan-yan;RUAN Yu;GU Xin;XIAO Guo-sheng(College of Biology and Food Engineering,Chongqing Three Gorges University,Wanzhou 404100,Chongqing,China)
出处
《林业科学研究》
CSCD
北大核心
2022年第4期130-142,共13页
Forest Research
基金
重庆市自然科学基金面上项目(cstc2020jcyj-msxmX0073)
重庆三峡学院博士引进项目(2014/09925401)
重庆市渝东北特色生物资源开发利用工程技术研究中心项目(1209/061800406)。
关键词
桑树
HD-ZipⅠ转录因子
鉴定
基因表达分析
Mulberry
HD-Zip Ⅰ transcription factor
identification
gene expression analysis
