摘要
近年来,微加工技术和组织工程学的进展推动了一种新型药物筛选和疾病建模平台的发展,即类器官芯片。这一平台结合类器官和器官芯片技术,成为人体器官体外建模的有潜力的方案。类器官芯片利用微流体装置模拟特定器官的生理环境,提供更动态和灵活的微环境,可模拟更完整的人体生物学。然而,缺乏功能性血管一直是该技术面临的主要挑战之一。血管化对于类器官的长期培养和体外建模至关重要,对于药物研发和个性化医疗方案也具有重要意义。笔者从体外构建血管化模型方法和类器官血管化的策略两方面就血管化类器官芯片构建的研究进展进行综述,为构建功能完善的血管化类器官芯片提供参考。
In recent years,advancements in microfabrication technology and tissue engineering have propelled the development of a novel platform known as organoid-on-a-chip for drug screening and disease modeling.This platform integrates organoids and organ-on-a-chip technologies,emerging as a promising approach for in vitro modeling of human organs.Organ-on-a-chip leverages microfluidic device to simulate the physiological environment of specific organs,offering a more dynamic and flexible setting that can mimic a more comprehensive human biological context.However,the lack of functional vasculature has remained a major challenge in this technology.Vascularization is crucial for the long-term cultivation and in vitro modeling of organoids,which is of great significance in drug development and personalized medical approaches.The authors reviewed the research progress in the construction of vascularized organoid-on-a-chip including the methods for constructing in vitro vascularized models,vascularization of organoids,etc,which may serve as a reference for the construction of fully functional vascularized organoid-on-a-chip.
作者
李彰杰
周晨阳
王晓林
Li Zhangjie;Zhou Chenyang;Wang Xiaolin(Department of Micro/Nano Electronics,School of Electronic Information and Electrical Engineering,Shanghai Jiao Tong University,Shanghai 200240,China;Institute of Medical Robotics,Shanghai Jiao Tong University,Shanghai 200240,China)
出处
《中华创伤杂志》
CAS
CSCD
北大核心
2024年第1期48-56,共9页
Chinese Journal of Trauma
基金
国家自然科学基金(31972929,62231025)。
关键词
类器官
新生血管化
生理性
多能干细胞
微流控芯片
Organoids
Neovascularization,physiological
Multipotent stem cells
Microfluidic chips
