摘要
目的:探讨结肠癌组织中circ_0011385和miR-330-3p表达情况,研究circ_0011385靶向miR-330-3p对结肠癌细胞(SW1116)恶性行为的影响。方法:RT-qPCR检测circ_0011385和miR-330-3p在结肠癌组织中表达情况,并分析circ_0011385和miR-330-3p表达水平与结肠癌患者临床病理特征的关系。皮尔森相关分析确定circ_0011385和miR-330-3p表达的相关性。circ_0011385和miR-330-3p的关系通过双荧光素酶报告实验来确定。将si-circ_0011385、si-NC、si-circ_0011385+anti-miR-NC、si-circ_0011385+anti-miR-330-3p分别转染SW1116细胞,Transwell、流式细胞术分析细胞迁移、侵袭和凋亡。结果:结肠癌组织中circ_0011385呈高表达,miR-330-3p呈低表达,二者的表达水平呈负相关关系(r=-0.8924)。circ_0011385和miR-330-3p表达水平与结肠癌患者年龄、肿瘤大小无相关性(P>0.05),与分化程度、TNM分期、淋巴结转移显著相关(P<0.05)。miR-330-3p是circ_0011385的靶基因。沉默circ_0011385表达后SW1116细胞凋亡率、miR-330-3p表达水平显著升高(P<0.05),细胞迁移数、侵袭数显著减少(P<0.05)。抑制miR-330-3p表达显著减弱沉默circ_0011385对SW1116细胞凋亡、迁移和侵袭的作用(P<0.05)。结论:沉默circ_0011385通过上调miR-330-3p表达来诱导结肠癌细胞凋亡并抑制其迁移和侵袭。
Objective:To investigate the expression of circ_0011385 and miR-330-3p in colon cancer tissues,and further study the effect of circ_0011385 targeting miR-330-3p on the malignant behavior of colon cancer cells(SW1116).Methods:RT-qPCR was performed to detect circ_0011385 and miR-330-3p expression in colon cancer tissues,and further analyze the relationship between the expression levels of circ_0011385 and miR-330-3p and the clinico-pathological characteristics of colon cancer patients.The relationship between circ_0011385 and miR-330-3p was confirmed by dual luciferase reporter assays.SW1116 cells were transfected with si-circ_0011385,si-NC,si-circ_0011385+anti-miR-NC,or si-circ_0011385+anti-miR-330-3p,respectively.Transwell and flow cytometry analysis were used to analyze cell migration,invasion and apoptosis.Results:In colon cancer tissues,circ_0011385 was highly expressed,while miR-330-3p was lowly expressed,and the expression levels of the two were negatively correlated(r=-0.8924).Circ_0011385 and miR-330-3p expression was not correlated with the age and tumor size of colon cancer patients(P>0.05),but it was significantly correlated with the degree of differentiation,TNM stage,and lymph node metastasis(P<0.05).MiR-330-3p is a target gene of circ_0011385.After silencing the expression of circ_0011385,the apoptosis rate of SW1116 cells and the expression level of miR-330-3p were significantly increased(P<0.05),and the number of cell migration and invasion was significantly reduced(P<0.05).Inhibition of miR-330-3p expression significantly reduced the effect of silencing circ_0011385 on SW1116 cell apoptosis,migration and invasion(P<0.05).Conclusion:Circ_0011385 is highly expressed,while miR-330-3p is lowly expressed in colon cancer.Silencing circ_0011385 induces cell apoptosis and inhibit the migration and invasion in colon cancer by up-regulating miR-330-3p expression.
作者
东金花
吴葆华
闫丹措
DONG Jinhua;WU Baohua;YAN dancuo(Qinghai Provincial People's Hospital, Qinghai Xining 810000, China)
出处
《河北医学》
CAS
2022年第6期909-913,共5页
Hebei Medicine
基金
青海省重点研发与转化计划项目,(编号:175110013106445)。
