摘要
目的研究结直肠癌(Colorectal cancer,CRC)组织中miRNA-371-5p的表达意义。方法以2011年1月-2013年1月山东省立第三医院胃肠外科收治的186例CRC患者为研究对象,取癌组织和癌旁组织,用RT-PRC法检测组织中miRNA-371-5p相对表达量。以miRNA-371-5p表达量的中位数为截点,将所有患者分为低表达量组和高表达量组,分析miRNA-371-5p表达量与患者预后的关系。用RT-PRC法检测结肠癌细胞(HT29、SW480、SW620和HCT116)和正常人肠上皮细胞-6(HIEC-6)中miRNA-371-5p相对表达量,分别用miRNA-371-5p模拟物和对照质粒转染SW620细胞,分为miRNA-371-5p及miRNA-NC组。克隆形成实验和CCK8法检测细胞增殖;Transwell实验检测侵袭和迁移;蛋白印迹法检测细胞增殖相关蛋白Ki67和PCNA表达水平。结果与癌旁组织比较,癌组织miRNA-371-5p相对表达量较低,差异有统计学意义(P<0.05)。与HIEC 6细胞比较,HT29、SW480、SW620和HCT116细胞的miRNA-371-5p相对表达量均较低,差异有统计学意义(P<0.05)。与miRNA-371-5p高表达组比较,低表达组生存时间较短,差异有统计学意义(P<0.05)。多因素分析发现,年龄(OR=2.157)、分化程度(OR=3.894)、淋巴结转移(OR=2.648)和miRNA-371-5p表达量(OR=1.169)是患者预后不良的独立危险因素(P<0.05)。转染miRNA-371-5p模拟物可抑制SW620细胞增殖、侵袭和迁移,并抑制Ki67和PCNA蛋白表达(P<0.05)。结论miRNA-371-5p在CRC组织中低表达,与生存预后有关。上调miRNA-371-5p可抑制CRC细胞增殖、侵袭和迁移。
Objective To study the expression and significance of miRNA-371-5p in colorectal cancer(CRC)tissue.Methods A total of 186 CRC patients were admitted to the hospital from January 2011 to January 2013 were selected as the research objects.The cancer tissues and the adjacent tissues were collected,and RT-PCR was used to detect miRNA-371-5p in the tissues with relative expression.Using median of miRNA-371-5p expression as cut-off point,all patients were divided into the low expression group and the high expression group.The relationship between miR⁃NA-371-5p expression and patient prognosis were analyzed.The relative expression of miRNA-371-5p in colon can⁃cer cells(HT29,SW480,SW620 and HCT116)and normal human intestinal epithelial cells(HIEC-6)were detected by RT-PRC,using miRNA-371-5p mimic and the control,respectively.The plasmids were transfected into SW620 cells and divided into miRNA-371-5p and miRNA-NC groups.Colony formation assay and CCK8 assay were used to detect cell proliferation;Transwell assay was used to detect invasion and migration;Western blotting was used to de⁃tect the expression levels of cell proliferation-related proteins Ki67 and PCNA.Results Compared with adjacent tis⁃sues,the relative expression of miRNA-371-5p in cancer tissues was lower,and the difference was statistically signifi⁃cant(P<0.05).Compared with HIEC-6 cells,the relative expression levels of miRNA-371-5p in HT29,SW480,SW620 and HCT116 cells were all lower,and the difference was statistically significant(P<0.05).Compared with the high expression group of miRNA-371-5p,the survival time of the low expression group was shorter,and the difference was statistically significant(P<0.05).Multivariate analysis showed that age(OR=2.157),differentiation(OR=3.894),lymph node metastasis(OR=2.648)and expression of microRNA-371-5p(OR=1.169)were independent risk factors for poor prognosis(P<0.05).Conclusion miRNA-371-5p is lowly expressed in CRC tissues and is associated with sur⁃vival prognosis.Up-regulation of miRNA-371-5p inhibit
作者
韩悦
王贲士
HAN Yue;WANG Benshi(Department of Gastrointestinal Surgery,the Third Hospital of Shandong Province,Jinan 250031,China;Department of Gastrointestinal Surgery,Shandong Cancer Hospital,Jinan 250106,China)
出处
《新疆医科大学学报》
CAS
2022年第6期615-622,共8页
Journal of Xinjiang Medical University
基金
山东省科技发展计划项目(2019GSF119036)。
关键词
结直肠癌
预后
增殖
侵袭
迁移
colorectal cancer
prognosis
proliferation
invasion
migration
