摘要
目的观察结肠癌组织及细胞中miR-30c-5p及Toll样受体4(Toll-like receptor4,TLR4)蛋白表达,探讨其与临床病理特征的关系及意义。方法采用前瞻性研究,选取2016年5月至2017年5月于中国医科大学肿瘤医院手术切除的结肠癌手术标本及配对癌旁正常组织标本30例,采用实时荧光定量聚合酶链反应(quantitative real-time polymerase chain reaction,qRT-PCR)检测miR-30c-5 mRNA的表达,采用蛋白质印迹(western blot,WC)法检测TLR4蛋白的表达,观察miR-30c-5p mRNA及TLR4蛋白在肿瘤不同TNM分期、分化程度及直径中的表达差异,Pearson Rank法进行miR-30c-5p及TLR4蛋白表达的相关性分析。结果在结肠癌组织中,miR-30c-5p表达(0.311±0.147)低于癌旁正常组织(0.881±0.266)(t=10.613,P<0.001)。TLR4蛋白在结肠癌组织(0.729±0.274)中表达高于癌旁正常组织(0.361±0.168)(t=6.310,P<0.001)。miR-30c-5p mRNA在结肠癌细胞株中表达(0.394±0.045、0.435±0.098、0.533±0.092、0.272±0.069)低于正常结肠上皮细胞(1.371±0.101)(t值分别为6.744、6.423、6.865、6.201,P均<0.001)。TLR4蛋白在结肠癌细胞株中表达(1.108±0.169、1.035±0.177、1.114±0.253、1.116±0.157)高于正常结肠上皮细胞(0.358±0.094)(t值分别为5.789、4.799、5.311、5.291,P均<0.001)。Pearson rank相关分析显示,在结肠癌组织中miR-30c-5p及TLR4蛋白表达量呈负相关(r=-0.487,95%CI:-0.721~-0.154,P<0.01)。miR-30c-5p随着TNM分期升高呈现下降趋势(F=31.406,P<0.001),随着肿瘤分化程度下降呈现下降趋势(F=9.960,P<0.001),随着肿瘤直径增大呈现下降趋势(F=10.267,P<0.001)。TLR4随着TNM分期升高呈现上升趋势(F=37.634,P<0.001)。TLR4随着肿瘤分化程度下降呈现上升趋势(F=38.027,P<0.001)。TLR4随着肿瘤直径增大呈现上升趋势(F=20.717,P<0.001)。结论miR-30c-5p在结肠癌中低表达,TLR4在结肠癌中高表达,二者与结肠癌TNM分期及肿瘤体积等恶性临床病理特征具有相关性。
Objective To observe the expression of miR-30c-5p and Toll-like receptor 4(TLR4)in colon cancer tissues and cells,and to explore their relationship with clinicopathological features.Methods As a prospective study,30 cases of colon cancer surgical specimens and matched normal tissue samples were selected from the Cancer Hospital of China Medical University from May 2016 to may 2017.The expression of miR-30c-5p mRNA was detected by quantitative real-time polymerase chain reaction(qRT-PCR),and the expression of TLR4 Protein was detected by western blot(WC).The expression differences of miR-30c-5p mRNA and TLR4 protein in different TNM stages,differentiation degrees and diameters were observed.The correlation between the expression of miR-30c-5p and TLR4 Protein was analyzed by Pearson Rank method.Results The expression of miR-30c-5p was lower in colon cancer tissues(0.311±0.147)than in adjacent normal colon tissues(0.881±0.266)(t=10.613,P<0.001).TLR4 protein was higher in colon cancer tissues(0.729±0.274)than in adjacent normal colon tissues(0.361±0.168)(t=6.310,P<0.001).Expression of miR-30c-5p was lower in colon cancer cell lines(0.394±0.045,0.435±0.098,0.533±0.092,0.272±0.069)than in normal colon epithelial cell line(1.371±0.101)(t value were 6.744,6.432,6.865 and 6.201,respectively;P<0.001).The expression of TLR4 protein was higher in colon cancer cell lines(1.108±0.169,1.035±0.177,1.114±0.253,1.116±0.157)than in normal colon epithelial tissues(0.358±0.094)(t value were 5.789,4.799,5.311 and 5.292,respectively;P<0.001).Pearson rank correlation showed that miR-30c-5p was negatively correlated with TLR4 protein expression(r=-0.487,95%CI:-0.721--0.154,P<0.01).MiR-30c-5p was decreased with TNM stage increasing(F=31.406,P<0.001),pathological differentiation degree decreasing(F=9.960,P<0.001),tumor diameter increasing(F=10.267,P<0.001).TLR4 was increasing with TNM stage increasing(F=37.634,P<0.001).TLR4 increased with the decrease of tumor differentiation(F=38.027.35,P<0.001).TLR4 increased with tumor di
作者
闫晓菲
赵广越
乔鋆
张继福
石刚
Yan Xiaofei;Zhao Guangyue;Qiao Yun;Zhang Jifu;Shi Gang(Department of Colorectal Surgery,Cancer Hospital of China Medical University&Liaoning Cancer Hospital﹠Institute,Shenyang 110042,China)
出处
《中国综合临床》
2021年第1期57-61,共5页
Clinical Medicine of China
基金
辽宁省自然科学基金(2020-ZLLH-42)。
