摘要
目的探讨血清miR-200a、miR-190联合检测在结直肠癌诊断及预后评估中的应用价值。方法选择2016年1月至2018年1月期间在昌江县人民医院接受手术治疗的63例结直肠癌患者作为结直肠癌组,于术前和术后3个月采集静脉血液标本,并随访1年统计复发情况;另选取35位同期健康体检者作为对照组,于体检当日采集血液标本。比较两组的血清miR-200a、miR-190表达水平及其与临床病理参数的关系,并比较结直肠癌组复发者与未复发者在术后3个月的血清miR-200a、miR-190表达水平的变化情况。采用受试者工作特征(ROC)曲线评价血清miR-200a、miR-190诊断结直肠癌以及预测术后复发的效能。结果与对照组相比较,结直肠癌组的血清miR-200a、miR-190表达水平均降低,TNM分期为Ⅲ~Ⅳ期者的miR-200a表达水平低于Ⅰ~Ⅱ期者,存在淋巴结转移者的miR-200a和miR-190水平低于无转移者,上述差异均有统计学意义(P均<0.05)。结直肠癌患者术后随访1年,复发16例,未复发47例,复发组术后3个月的血清miR-200a、miR-190表达水平均低于未复发组,差异有统计学意义(P<0.05)。术前血清miR-200a、miR-190联合诊断结直肠癌的ROC曲线下面积(AUC)大于单指标诊断,术后3个月血清miR-200a、miR-190联合预测结直肠癌患者术后复发的AUC大于单指标预测。结论结直肠癌患者的血清miR-200a、miR-190表达水平下降,可作为结直肠癌早期诊断及预测术后复发的标志物,两者联合检测可提高诊断及预测的敏感度和特异度。
Objective This paper attempted to investigate the value of combined detection of serum miR-200 a and miR-190 in the diagnosis and prognosis of colorectal cancer.Methods Sixty-three patients with colorectal cancer who underwent surgery in the People′s Hospital of Changjiang County from January 2016 to January 2018 were enrolled in the colorectal cancer group whose venous blood samples were collected before and 3 months after surgery and the recurrence was followed up for 1 years.In the same period,35 healthy persons were selected as the control group,whose blood samples were collected on the day of the physical examination.The expressions of serum miR-200 a and miR-190 and their relationship with clinicopathology were compared between the two groups,and the changes of expressions of serum miR-200 a and miR-190 in patients with recurrence of colorectal cancer and those without recurrence at 3 months after operation were compared.The efficacy of the expressions of serum miR-200 a and miR-190 in the diagnosis of colorectal cancer was evaluated using ROC curves and the postoperative recurrence of colorectal cancer was predicted.Results The expressions of serum miR-200 a and miR-190 in the colorectal cancer group were lower than those in the healthy control group.The expression of miR-200 a in patients with colorectal cancer in the TNM stage(stageⅢ-Ⅳ)was lower than that in stageⅠ-Ⅱwhile those of miR-200 a and miR-190 in patients with lymph node metastasis of colorectal cancer were lower than those without metastasis.The above differences were statistically significant(P<0.05).The patients with colorectal cancer were followed up for 1 years.There were 16 cases of recurrence and 47 cases of non-recurrence.The expressions of serum miR-200 a and miR-190 in the recurrence group were lower than those in the non-recurrence group 3 months after operation(P<0.05).The expression of preoperative serum miR-200 a combined with that of miR-190 for diagnosis of colorectal cancer under the ROC curve area(AUC)is greater than
作者
王胜
吴英光
何孟胜
WANG Sheng;WU Yingguang;HE Mengsheng(Department of General Surgery,the People's Hospital of Changjiang County,Hainan 572700,China)
出处
《国际消化病杂志》
CAS
2019年第4期286-290,共5页
International Journal of Digestive Diseases
