摘要
研究不同干燥方式对艾草品质的影响,采用HPLC法、Eclipse Plus C;(250 mm×4.6 mm,5 m)色谱柱,以0.5%磷酸水溶液(A)-甲醇(B)为流动相梯度洗脱,流速1 mL/min,柱温30℃,检测波长320 nm,进样量10μL,建立晒干、阴干和热风干燥3种不同方式干燥艾草的HPLC指纹图谱,用聚类分析、主成分分析等化学计量学方法进行评价。结果表明,3种方式干燥的12个艾草样品有27个色谱峰,其中25个为共有峰;并指认了5个色谱峰,分别为绿原酸、异绿原酸A、木犀草苷、芦丁和表儿茶素。热风干燥艾草中总黄酮含量达21.44 mg/g,晒干干燥艾草中含量仅为13.02 mg/g。热风干燥的艾草DPPH自由基和羟基自由基清除率最高,分别是87.77%和77.38%,晒干艾草的总还原力最强。热风干燥的艾草可较好地保持有效成分和抗氧化活性,可在新鲜艾草中进行广泛推广。
In order to explore the effect of different drying techniques on the quality of Artemisia argyi,HPLC was performed on an Eclipse Plus C;column( 250 mm × 4. 6 mm,5 m) by a gradient elution using 0. 5% phosphoric acid( A) and methanol( B). The flow rate was 1 m L/min and column temperature was 30 ℃ . The detection wavelength was 320 nm and the injection volume was 10 μL. Cluster analysis( CA) and principal component analysis( PCA) were used to evaluate the HPLC fingerprints of Artemisia argyi dried with three different ways: solar drying,shade drying and hot air drying. The results indicated that a total of 27 chromatographic peaks were found in the 12 samples of Artemisia argyi dried by solar drying( SD),shade drying( SHD) and hot air drying( HAD) techniques,respectively,among which 25 were common peaks and 5 chromatographic peaks were identified,namely chlorogenic acid,isochlorogenic acid A,galuteolin,luteolin,epicatechin. Total flavonoid content in samples of HAD were 21. 44 mg/g and the lowest was 13. 02 mg/g in samples of SHD. The samples dried by HAD had strongest scavenging abilities of DPPH free radical and hydroxyl radical up to 87. 77% and 77. 38%,respectively. However,samples dried by SD had the strongest of total reducing power. It can be concluded that hot air drying could maintain bioactive components and antioxidant activities of Artemisia argyi which could be widely promoted in the drying process of fresh Artemisia argyi.
作者
于士军
程铭
周怡倩
王伟
徐志香
徐馨怡
刘金月
YU Shijun;CHENG Ming;ZHOU Yiqian;WANG Wei;XU Zhixiang;XU Xinyi;LIU Jinyue(School of Biology and Food Engineering,Chuzhou University,Chuzhou 239000,China;Administration for Quality and Safety of Agricultural Products in Xuancheng,Xuancheng 242000,China)
出处
《宿州学院学报》
2021年第12期35-41,共7页
Journal of Suzhou University
基金
安徽省教育厅高校优秀青年骨干人才国内研修项目(GXGNFX2020105)
安徽省教育厅一流本科人才示范引领基地(2019rcsfjd082)
国家级大学生创新创业训练项目(201910377026,202010377032)
滁州学院大学生创新创业训练项目(2019CXXL017,2020CXXL032)。
关键词
艾草
干燥方法
HPLC指纹图谱
抗氧化活性
Artemisia argyi
Drying technique
HPLC fingerprint
Antioxidant activity
