摘要
The antiviral substances of walnut leaves were extracted by different solvents and methods, and its antiviral activities were determined. The results showed that antiviral substance activity is dissimilar based on extracts from walnut leaves with different solvent and methods. Inhibition rate on TMV of the extract with 95% (V/V) ethanol is the best, 100%; Optimum condition of the cold soak are: 95% (V/V) ethanol is used as solvent, the ratio of material to solvent is 20/100 (1∶10), soak time is 18 h. The inhibition rate of the extract to TMV infecting Nicotiana glutinosa is 100% under optimum condition.
The antiviral substances of walnut leaves were extracted by different solvents and methods, and its antiviral activities were determined. The results showed that antiviral substance activity is dissimilar based on extracts from walnut leaves with different solvent and methods. Inhibition rate on TMV of the extract with 95% （V/V） ethanol is the best, 100% ; Optimum condition of the cold soak are： 95% （V/V） ethanol is used as solvent, the ratio of material to solvent is 20/100 （1：10）, soak time is 18 h. The inhibition rate of the extract to TMV infecting Nicotiana glutinosa is 100% under optimum condition.
出处
《林业科学》
EI
CAS
CSCD
北大核心
2007年第7期148-151,共4页
Scientia Silvae Sinicae
基金
国家自然科学基金(30571494)
中国博士后基金(2004035689)
陕西省科技攻关项目(2004K03-G3)
西北农林科技大学优秀人才专项基金(04ZR005)。
关键词
核桃
烟草花叶病毒
提取方法
walnut
tobacco moscaic virus （TMV）
extracted method
