摘要
从广西北部湾防城港红树林筛选到1株高产胞外多糖的微藻GF02,结合形态学与分子生物学鉴定为小球藻属Chlorella Sorokiniana。初步表征结果表明GF02胞外粗多糖以α-型吡喃糖为主,通过响应面法优化GF02产胞外多糖的条件,实验表明,GF02产胞外多糖最佳培养条件为NaNO_(3)2.1 g/L,Na_(2)CO_(3)0.02 g/L,MgSO_(4)0.086 g/L,K_(2)HPO_(4)0.06 g/L,在此条件下,其胞外多糖积累量为216.268 mg/L,是优化前的1.60倍。对胞外多糖抗氧化活性进行了初步的研究,抗氧化活性结果表明,GF02粗多糖对DPPH清除率达到36.53%,对羟基自由基清除率达到67.33%。该研究为小球藻多糖的制备和进一步开发利用提供了实验基础。
A microalgae strain with high exopolysaccharides production isolated from mangrove in Fangchenggang city,Beibu Gulf region,Guangxi province was named as GF02.GF02 was identified as Chlorella sorokiniana according to morphology and molecular biology analysis.Preliminary characterization results showed that the main component of the extracellular polysaccharide wasα-pyranose.Response surface method was used to optimize the culture conditions of GF02 for the extracellular polysaccharide production.The results showed that the optimal culture condition for GF02 to produce exopolysaccharides were NaNO_(3)2.1 g/L,Na_(2)CO_(3)0.02 g/L,MgSO_(4)0.086 g/L and K_(2)HPO_(4)0.06 g/L.Under these conditions,the accumulation of extracellular polysaccharides was 216.268 mg/L,which was 1.60 times of that before optimization.The results of antioxidant activity analysis showed that the eliminating rate of GF02 exopolysaccharides on DPPH and hydroxyl radicals were up to 36.53%and 67.33%,respectively.This study would provide experimental basis for the preparation and further utilization of Chlorella polysaccharides.
作者
吴思伟
李思雨
孙寒
刘红全
何秀苗
黄莹
吴嘉惠
黄柳媚
龙寒
WU Siwei;LI Siyu;SUN Han;LIU Hongquan;HE Xiumiao;HUANG Ying;WU Jiahui;HUANG Liumei;LONG Han(Guangxi Key Laboratory of Polysaccharide Materials and Modification,College of Marine and Biotechnology,Guangxi University for Nationalities,Nanning 530006,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2021年第24期193-200,共8页
Food and Fermentation Industries
基金
广西自然科学基金项目(2018GXNSFAA294032)
广西民族大学研究生教育创新计划项目(gxun-chxps201919)。
关键词
小球藻
胞外多糖
分离鉴定
培养条件
抗氧化活性
Chlorella sorokiniana
extracellular polysaccharide
isolation and identification
culture condition
antioxidant activity
