摘要
【目的】明确多孢木霉HZ-31菌株对藜植株体内生理生化特性的影响.【方法】利用酶活力测试法测定了HZ-31菌株接种藜植株后体内丙二醛(MDA)含量、过氧化氢酶(CAT)活性、过氧化物酶(POD)活性、多酚氧化酶(PPO)活性、苯丙氨酸解氨酶(PAL)活性、超氧化物歧化酶(SOD)活性、可溶性糖含量和叶绿素含量.【结果】多孢木霉HZ-31菌株接种藜后其体内MDA和可溶性糖含量及CAT、PPO和SOD的活性较对照明显增加,且呈上升趋势,增加量最高分别达到67.91%、69.37%、81.88%、37.68%和8.12%,7 d后除MDA呈下降趋势外,其他指标均呈增加趋势;叶绿素含量第3天后低于对照,呈下降趋势;POD活性呈平稳变化趋势且始终高于对照;而PAL活性呈先上升再下降的趋势.【结论】多孢木霉HZ-31侵染藜后,藜防御酶体系被激活,抗侵染性增强.
【Objective】To clarify the effect of Trichoderma multisporum HZ-31 strain on the physiological and biochemical characteristics of Chenopodium album.【Method】The plants of C.album were inoculated with HZ-31,the determination was conducted on the content of soluble sugar,chlorophyll and malondialdehyde(MDA),and the activity of catalase(CAT),peroxidase(POD),polyphenol oxidase(PPO),phenylalanine ammonia-lyase(PAL)and superoxide dismutase(SOD).【Result】The contents of MDA and soluble sugars and the activities of CAT,PPO,and SOD in the inoculated plants were significantly increased compared with the control,and showed an upward trend.The highest increase in the inoculated plants reached 67.91%,69.37%,81.88%,37.68%and 8.12%,respectively.Except MDA content showed a downward trend after 7 days,the other index showed an increasing trend.The chlorophyll content was lower than that of the control after the 3rd day,showed a downward trend;POD activity showed a steady change trend and was always higher than that of the control;And the PAL activity showed a trend of increasing first and then decreasing.【Conclusion】It is suggested that T.multisporum HZ-31 infection causes significantly defense response in C.album.
作者
蔺泽荣
朱海霞
LIN Zerong;ZHU Haixia(Qinghai Academy of Agricultural and Forestry Sciences,Xining Crop Pest Scientific Observation and Experimental Station,Ministry of Agriculture,Key Laboratory of Comprehensive Control of Agricultural Pests,Xining 810016,China)
出处
《甘肃农业大学学报》
CAS
CSCD
2021年第4期120-125,共6页
Journal of Gansu Agricultural University
基金
青海省科技厅应用基础研究项目(2019-ZJ-7057)
青海省农业有害生物综合治理重点实验室项目(2021-ZJ-Y08).
关键词
多孢木霉HZ-31
侵染
藜
防御酶
Trichoderma polysporum HZ-31
infection
Chenopodium album
defense enzymes
