摘要
在盆栽试验中分别接种3株长枝木霉菌株Trichoderma longibrachiatum MF-1、MF-2和MF-3,以不接种木霉菌处理作为对照,研究木霉菌接种对土著AM真菌和黄瓜幼苗生长的影响。结果表明,菌株MF-1和MF-2显著提高了AM真菌侵染率和根外菌丝密度,与对照相比,AM真菌侵染率分别提高了26.85%和54.66%,根外菌丝密度分别是对照的3.94和3.76倍。接种菌株MF-2使植株地上部生物量显著提高了39.07%。菌株MF-3显著提高土壤pH和土壤有效磷含量。Pearson相关分析发现,添加木霉菌后,AM真菌侵染率与根外菌丝密度和孢子密度均呈显著正相关关系,土壤pH与植株地上部生物量和土壤有效磷含量均呈显著正相关关系。研究表明,3株长枝木霉与土著AM真菌的联合作用效果有明显差异,菌株MF-1和MF-2显著促进AM真菌生长,菌株MF-2更有利于黄瓜幼苗生长,而菌株MF-3主要改善土壤pH和有效磷含量。将几种木霉菌复合应用,有助于达到联合促生和改善土壤环境的综合效果。
A pot experiment was conducted to investigate the effect of Trichoderma and indigenous arbuscular mycorrhizal(AM) fungi on cucumber seedling growth, using three strains of Trichoderma longibrachiatum,MF-1, MF-2 and MF-3, as inocula. Uninoculation treatment was set up as experimental control. The results showed that significant increases of AM fungal colonization in root and hyphal density in rhizosphere soil were found in the treatments inoculated with MF-1 and MF-2. In comparison with the control, the AM fungal colonization rate in treatments inoculated with MF-1 and MF-2 increased by 26.85% and 54.66% respectively,and the rhizosphere hyphal density of treated seedlings was 3.94 and 3.76 times respectively as high as the untreated control. The shoot dry mass of cucumber seedlings inoculated with MF-2 showed a 39.07% increase as compared with the control. The soil pH and available P of seedlings inoculated with MF-3 were higher than those of untreated seedlings. Pearson correlation analysis indicated that AM fungal colonization rate of seedlings was positively related to the hyphal density and spore density in rhizosphere soil, and soil pH was positively related to the shoot dry mass and soil available P. These results suggest that the AM fungal growth is significantly affected by the inoculation of MF-1 and MF-2, and MF-2 has a greater impact on the plant growth than MF-1, while MF-3 tends to improve soil pH and available P. Compound inocula of Trichoderma are helpful for promoting growth and soil environment of cucumber seedlings.
作者
杨榕
宋春晖
李晓光
黄志勇
YANG Rong;SONG Chun-Hui;LI Xiao-Guang;HUANG Zhi-Yong(Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering,Tianjin Institute of Industrial Biotechnology,Chinese Academy of Sciences,Tianjin 300308,China;Key Laboratory of Mineral Resources in Western of Gansu Province,Earth Science College of Lanzhou University,Lanzhou,Gansu 730000,China;Binzhou Engineering Technology Research Center for High Salt Wastewater Treatment(Preparatory Department)of Befar Group,Binzhou,Shandong 256602,China)
出处
《菌物学报》
CAS
CSCD
北大核心
2019年第2期178-186,共9页
Mycosystema
基金
国家自然科学基金(41807395)
天津市科技计划项目(15PTCYSY00020)
甘肃省西部矿产资源重点实验室开放课题(WCRMGS-2014-02)~~
关键词
长枝木霉
AM真菌
黄瓜
联合促生
Trichoderma longibrachiatum
AM fungi
cucumber
promoting growth
