摘要
目的:采用网络药理学方法探讨巴戟天抗风湿的物质基础及作用机制。方法:通过中药系统药理学平台(TCMSP)获取巴戟天有效成分及其作用靶点,通过GeneCards平台获取类风湿性关节炎疾病作用靶点;利用Uniprot在线平台对靶点名称进行校正,取有效成分靶点与疾病交集靶点交集,并通过String在线平台生成蛋白互作(PPI)图;运用R语言程序包对靶点基因进行基因本体论(GO)生物过程和KEGG信号通路富集分析;通过Cytoscape3.7.1软件为巴戟天有效成分、靶点及信号通路绘制网络互作图。结果:获得巴戟天潜在有效成分(DL≥0.18)共62个,其中可能的关键成分为2-羟基-3-甲基蒽醌、1-甲氧基-2-羟基蒽醌、洋商陆素A、美商陆酚A、大黄素甲醚、茜草素-1-甲醚、1-羟基-2-甲氧基蒽醌;巴戟天有效成分对应靶点37个,类风湿性关节炎疾病作用靶点(gifts≥10)3949个,两者交集靶点22个;PPI网络中关键靶点为NR3C1、PTGS2、CASP3、ACHE、SLC6A4、DRD2、PGR、OPRM1;GO功能分析主要集中在以类固醇激素受体活性、G蛋白偶连的胺受体活性、核受体活性、配体激活转录因子活性、儿茶酚胺结合、药物结合、类固醇结合、神经递质受体活性等生物功能,KEGG通路18条,可能主要通过调控神经活性配体-受体相互作用信号通路发挥作用。结论:研究初步探讨了巴戟天治疗类风湿关节炎的潜在化学成分、作用靶点和信号通路,为下一步深入研究其药效物质基础及效应机制提供依据。
Objective:To explore the anti-rheumatic material basis and mechanism of Morinda officinalis using network pharmacology.Methods:Obtain the active ingredients and targets of Morinda officinalis through the Traditional Chinese Medicine System Pharmacology Platform(TCMSP),and obtain rheumatoid arthritis disease targets through the GeneCards website.Correct the target name through the Uniprot website,take the effective component target and the disease intersection target to intersect,and use the String online platform to generate a protein interaction(PPI) map.Use R language program package to carry out gene ontology(GO) biological process and KEGG signal pathway enrichment analysis on target genes.The Cy to scape 3.7.1 software was used to draw network interaction maps for the active ingredients,targets and signal pathways of Morinda officinalis.Results:A total of 62 ingredients of Morinda officinalis(DL≥0.18) were obtained.It is possible that the main effective ingredients for anti-rheumatoid arthritis are 2-hydroxy-3-methylanthraquinone(MOL001649) and 1-methoxy-2-Hydroxyanthraquinone(MOL009514),americanin A(MOL0095 37),Americanol A(MOL0095 3 8),Physcion(MOL000476),alizarin-1-methyl ether(MOL009536),1-hydroxy-2-Methoxyanthraquinone(MOL006147).The active ingredients of Morinda officinalis correspond to 37 targets,3949 targets for rheumatoid arthritis disease(gifts≥10),and22 targets for the intersection of the two.The key targets in the PPI network are NR3 C1,PTGS2,CASP3,ACHE,SLC6 A4,DRD2,PGR,OPRM1.GO functional analysis mainly focuses on steroid hormone receptor activity,G protein-coupled amine receptor activity,nuclear receptor activity,ligand activated transcription factor activity,catecholamine binding,drug binding,steroid binding,neurotransmitter receptor activity and other biological functions.18 KEGG pathways focus on regulating neural active ligand-receptor interaction signaling pathways.Conclusion:The study initially explored the potential mechanism of Morinda officinalis in the treatment of rheumatoid ar
作者
廖慧君
张叶金
王钰
何任贤
谢利霞
赖正权
LIAO Hui-jurt;ZHANG Ye-jin;WANG Yu;HE Ren-xian;XIE Li-xia;LAI Zheng-quan(Huazhong University of Science and Technology Union Shenzhen Hospital(the 6^(th)affiliated hospital of Shenzhen University),Shenzhen,Guangdong 518052;Shenzhen University General Hopital,Shenzhen,Guangdong 518055)
出处
《按摩与康复医学》
2021年第15期47-52,共6页
Chinese Manipulation and Rehabilitation Medicine
基金
广东省基础与应用基础研究基金,编号:2019A1515010815
深圳市科技计划项目,编号:JCYJ20190808114807611
南山区科技计划项目,编号:医疗卫生类2020127。
关键词
类风湿性关节炎
巴戟天
网络药理学
物质基础
作用机制
rheumatoid arthritis
Morinda officinalis
network pharmacology
material basis
mechanism
