摘要
目的:比较巴戟天不同炮制品及提取部位的祛风湿作用,探讨其在细胞因子水平上的作用机制。方法:大鼠左后足跖皮内注射弗氏完全佐剂制备AA模型,分别灌胃给予巴戟天、巴戟肉、盐巴戟天、制巴戟天4种炮制品和巴戟天乙酸乙酯萃取部位、正丁醇萃取部位、剩余水部位和总多糖部位,以雷公藤多苷片为对照,在不同的时间点检测大鼠继发侧关节肿胀度;致炎28 d处死大鼠,采用ELISA法检测大鼠血清炎症细胞肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、白细胞介素-2(IL-2)、干扰素-γ(IFN-γ)的水平。结果:与模型组比较,盐巴戟天及巴戟天乙酸乙酯部位、巴戟天正丁醇部位能明显减轻AA大鼠足肿胀度,降低异常升高的血清TNF-α、IL-1β、IL-6、INF-γ水平;而巴戟天、制巴戟天、巴戟天总多糖和巴戟天剩余水部位作用不明显。结论:盐巴戟天、巴戟天正丁醇部位和巴戟天乙酸乙酯部位对AA大鼠各项指标改善作用显著,效果优于巴戟天、制巴戟天、巴戟天总多糖和巴戟天剩余水部位,其作用机制可能与抑制促炎因子TNF-α、IL-1β、IL-6、INF-γ的产生有关。
Objective：To study the therapeutic effects of different processed products and extracts of Morinda officinalis root in rat adjuvant-induced arthritis（AA） model,and to explore the possible mechanism. Methods：AA model in SD rats was established, then the arthritic rats were randomly divided into seven groups such as model ,Triptetrygium Glycosides Tablet（ TGT）, morinda with xylem, morinda,salt-steamed morinda,licorice-processed morinda, morinda EtOAc extract, morinda n-BuOH extract, H2O residue, total polysaccha- rides and control groups. The paw edema and arthritis indexes were observed, and the contents of tumor necrosis factor-α（TNF-α） ,in- terleukin-1β（ IL-1β）, IL-6,IL-2 and INF-γ in AA rats serum were detected by ELISA. Results ：Morinda processed by salt and n-BuOH extract could reduce paw swelling and levels of TNF-α, IL-1β, IL-6 and INF-γ in AA rats serum, which followed by morinda EtOAc extract. The differences were significant compared with the model group （ P 〈 0. 05 or P 〈 0. 01 ）. Conclusion： Salt-steamed morinda and morinda n-BuOH extract have the best curative effect, followed by morinda EtOAc extract. Reducing TNF-α, IL-1β, IL-6 and INF-γ con- tents in serum may be the mechanism of drug treatment of rheumatoid arthritis.
出处
《中药材》
CAS
CSCD
北大核心
2015年第8期1626-1629,共4页
Journal of Chinese Medicinal Materials
基金
国家自然科学基金青年基金(81001635)
国家发改委行业专项(201107007)
关键词
巴戟天
炮制
佐剂性关节炎
细胞因子
足肿胀
Morinda officinalis How
Processing
Adjuvant-induced arthritis
Cytokine
Paw swelling
