摘要
研究采用分光测色仪获取不同基原大黄药材粉末的颜色特征信息,通过统计分析探究基于色度学原理对大黄药材基原进行快速鉴别的可行性。秩相关分析结果显示:a^(*)(P<0.01),b^(*)(P<0.01)与药材基原呈极显著相关,可作为判别大黄基原的2个重要参数,其中a^(*)越大表示药材粉末越偏向于红色;b^(*)越大表示药材粉末颜色越偏向于黄色。通过Fisher判别分析,建立了不同基原大黄的线性判别函数,分别为唐古特大黄=40.666a^(*)+0.019b^(*)-213.303,掌叶大黄=34.121a^(*)+0.061b^(*)-151.770,药用大黄=28.071a^(*)+0.113b^(*)-104.604 3,交叉验证判别符合率均达到95%以上,其中唐古特大黄和药用大黄的判别率达到100%;此外,采用百分位数法,对3个不同基原大黄双侧90%参考值范围进行分析,结果分别为唐古特大黄a^(*)(10.236 5~10.604 7),b^(*)(32.294 8~34.841 7);掌叶大黄a^(*)(8.602 7~8.770 0),b^(*)(27.534 8~28.968 6);药用大黄a^(*)(6.825 7~7.464 3),b^(*)(21.001 6~27.716 4)。该研究表明采用秩相关分析和Fisher判别分析的方法在一定范围内对大黄药材基原判别具有一定可行性,为大黄药材基原鉴别提供一定理论依据,同时也为其他多基原中药的鉴定提供了一种新的方法及思路。
The color characteristic information of Rhei Radix et Rhizoma powder was obtained by spectrophotometer, the feasibility of rapid identification of Rhei Radix et Rhizoma origin based on chromaticity value was studied by statistical analysis. The results of rank correlation analysis showed that a^(*)(P<0.01), b^(*)(P<0.01) had significantly correlation with the origin of medicinal herbs, which could be used as two important parameters to distinguish the origin of Rhei Radix et Rhizoma, the larger the a^(*) value, the more red the powder color,and the greater the b^(*) value, the more yellow the powder color. Meanwhile, through Fisher discriminant analysis, the linear discriminant functions of different genus Rhei Radix et Rhizoma were established, which was Rheum tanguticum=40.666a^(*)+0.019b^(*)-213.303, Rh. palmatum=34.121a^(*)+0.061b^(*)-151.770, Rh. officinale=28.071a^(*)+0.113b^(*)-104.604 3, the coincidence rate of cross-validation was over 95%, among them, the discriminant rate of Rh. tanguticum and Rh. officinale reached 100%;In addition, using the percentile method to analyze the 90% reference value range of three different origin of Rhei Radix et Rhizoma, as a result, Rh. tanguticum a^(*)(10.236 5-10.604 7), b^(*)(32.294 8-34.841 7);Rh. palmatum a^(*)(8.602 7-8.770 0), b^(*)(27.534 8-28.968 6), and Rh. officinale a^(*)(6.825 7-7.464 3),b^(*)(21.001 6-27.716 4). According to this study, rank correlation analysis and Fisher discriminant analysis are feasible to distinguish the base of Rhei Radix et Rhizoma in a certain range, and provide some theoretical basis for the identification of Rhei Radix et Rhizoma. It also provides a new method and idea for the identification of other multi-base Chinese medicine.
作者
祁晓娟
李欣
卓冰雨
魏胜利
赵婷
QI Xiao-juan;LI Xin;ZHUO Bing-yu;WEI Sheng-li;ZHAO Ting(School of Chinese Pharmacy,Beijing University of Chinese Medicine,Beijing 102488,China;Engineering Research Center of Good Agricultural Pnutice for Chinese Crude Drugs of Ministry of Education,Beijing 100102,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2021年第4期902-906,共5页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(31570331,31170307)
国家重点研发计划项目(2017YFC1700702)。
关键词
颜色
大黄
基原
判别
color
Rhei Radix et Rhizoma
origin
discrimination
