摘要
目的在检测实验室能力验证样品时,通过多种方法进行比较,提高检测能力和结果准确性。方法菌落总数依据国标方法,在相同条件下由不同操作人员重复测定共10次,通过测量结果的不确定度的评定确定置信区间。大肠菌群通过3种方法计数,用大肠杆菌/大肠菌群测试片对大肠埃希氏菌进行快速定性判定。金黄色葡萄球菌通过不同培养基、涂布量、接种方法的7种不同组合方法进行检测。结果本次能力验证结果报告菌落总数结果为23000 CFU/mL、大肠菌群结果为10000 CFU/mL、金黄色葡萄球菌结果为12000 CFU/mL、大肠埃希氏菌检出。4项测试结果均为满意。结论通过多种方法同步进行,能有效提高检测能力和结果准确性。
Objective To improve the test ability and accuracy of the results through a variety of methods to compare,while the test of laboratory ability to verify the samples.Methods Based on national standards,the test of the total number of colonies was repeated ten times by different operators under the same conditions,and the confidence interval was determined by the evaluation of the uncertainty of the measurement results.Escherichia coli/coliform test tablets were used to quickly determine the coliform bacteria by counting the coliform bacteria in three ways.Staphylococcus aureus was detected by seven different combinations of different media,coating amount and inoculation methods.Results The proficiency test results were reported as colony count 23000 CFU/mL,coliform 10000 CFU/mL,Staphylococcus aureus 12000 CFU/mL,and E.coli was detected.The results of four tests were satisfied.Conclusion The detection ability and the accuracy of the results can be improved effectively by means of multiple synchronous methods.
作者
赵丽
申玉金
胡宝翠
刘建洋
ZHAO Li;SHEN Yu-Jin;HU Bao-Cui;LIU Jian-Yang(Shandong Standard Inspection Technology Co.,Ltd.,,Jinan 250100,China)
出处
《食品安全质量检测学报》
CAS
北大核心
2021年第1期320-325,共6页
Journal of Food Safety and Quality
