摘要
为探索致病疫霉游动孢子的最佳制备条件,将致病疫霉分别培养7~12 d后向其中加入Petri’s溶液,收集孢子囊并统计其产量,之后收集不同培养时间的孢子囊,分别采用5种不同低温诱导组合方法(M1:10℃静置30 min后18℃静置30 min;M2:4℃静置30 min后10℃静置90 min;M3:4℃静置30 min后10℃静置60 min;M4:4℃静置3 h;M5:4℃静置2 h后18℃静置1 h)制备游动孢子,研究不同培养时间及不同制备方法对游动孢子产量的影响。结果表明,随着培养时间的延长,致病疫霉孢子囊产量先迅速增加,后增加速度放缓直至基本不再增加;用不同制备方法得到的游动孢子产量随着孢子囊培养日龄的增加均呈先升高后降低的趋势,用培养9 d收获的致病疫霉孢子囊制备游动孢子时游动孢子产量最高,且不同制备方法得到的游动孢子产量差异不显著。综上,如要快速大量制备致病疫霉游动孢子,可用培养9 d的致病疫霉孢子囊在10℃下静置30 min后再在18℃下静置30 min,如不考虑制备时间,可用培养9 d的致病疫霉孢子囊在4℃静置2 h后再在18℃静置1 h,可获得最大量游动孢子;孢子囊产量与游动孢子产量并不完全呈正相关。
Phytophthora infestans is the pathogen of potato late blight,which poses a serious threat to potato production.In order to explore the optimal preparation conditions for the zoospores of P.infestans,after cultivation for 7—12 days,Petri’s solution was added to cultures,and sporangia was collected and counted.Five different low-temperature induction methods were used to prepare zoospores,and the effects of different culture time and different preparation methods on zoospore yield were studied.The low-temperature induction methods were as follows:M1,the sample was stood at 10℃for 30 min and then at 18℃for 30 min;M2,the sample was stood at 4℃for 30 min and then at 10℃for 90 min;M3,the sample was left at 4℃for 30 min and then at 10℃for 60 min;M4,the sample was stood at 4℃for 3 h;M5,the sample was stood at 4℃for 2 h and then at 18℃for 1 h.The results showed that with the extension of the cultivation time,the yield of sporangia increased rapidly first and then slowed down,finally basically stopped.As the cultivation time of sporangia increased,the zoospore yields obtained by different preparation methods increased first and then decreased.The yield of zoospores was the highest when sporangia were harvested from 9 day culture and there was no significant difference among different preparation methods.In summary,to quickly obtain zoospores in large quantities,the sporangia of P.infestans cultured for 9 days was stood at 10℃for 30 min and then at 18℃for 30 min.Or regardless of the preparation time,the 9-day-old sporangia was stood at 4℃for 2 h and then at 18℃for 1 h to obtain the largest number of zoospores.The yield of sporangia is not completely positively correlated with the yield of zoospores.
作者
杨祝强
王洪洋
唐唯
李灿辉
刘晶
YANG Zhuqiang;WANG Hongyang;TANG Wei;LI Canhui;LIU Jing(Yunnan Key Laboratory of Potato Biology,Yunnan Normal University,Kunming 650500,China;Key Laboratory of Potato Biology in Universities of Yunnan Province,Yunnan Normal University,Kunming 650500,China)
出处
《河南农业科学》
北大核心
2021年第3期103-108,共6页
Journal of Henan Agricultural Sciences
基金
云南省科技厅基础研究专项(202001AU070091)
云南省教育厅科学研究基金项目(2019J0067)
云南师范大学2018年博士科研启动项目(07800205020503005)。
关键词
致病疫霉
游动孢子
产量
培养时间
低温诱导
Phytophthora infestans
Zoospores
Yield
Cultivation time
Low-temperature induction