摘要
目的探究回回甘松饮(Hui⁃hui Gan⁃song Yin,HGY)对糖尿病肾病(diabetic nephropathy,DN)大鼠肾小球的保护作用。方法随机选取10只大鼠作为空白对照组(NC)。选用高脂饲料喂养SD大鼠联合链脲佐菌素的方法复制大鼠早期DN模型,DN模型复制成功的大鼠随机分为:DN模型组(DN)、格列喹酮组(DN+Gli,10 mg/kg)及回回甘松饮高剂量组(DN+HH,10 g/kg)、回回甘松饮低剂量组(DN+HL,5 g/kg),每组10只。采用实时荧光定量PCR方法检测肾组织中miR⁃192、靶基因Zeb2及TGF⁃β1/Smads信号通路中TGF⁃β1、Smad3、Smad7 mRNA的表达,采用Western blot方法检测所对应蛋白的表达。结果与空白对照组比较,模型组大鼠肾组织TGF⁃β1、Smad3蛋白及miR⁃192、TGF⁃β1、Smad3 mRNA表达水平显著升高(P<0.01),而Smad7、SIP1蛋白及Zeb2和Smad7 mRNA表达水平显著降低(P<0.01);与模型组比较,给予HGY及格列喹酮干预后,大鼠TGF⁃β1及miR⁃192、TGF⁃β1、Smad3 mRNA水平显著下降(P<0.01),Smad7、S1P1蛋白及Zeb2、Smad7 mRNA表达水平显著升高。结论HGY在DN大鼠肾脏中可能通过调控miR⁃192介导的TGF⁃β1/Smads信号通路改善肾脏细胞外基质堆积,从而起到保护早期DN大鼠肾脏的作用。
Objective To investigate the protective effect of Hui⁃Hui Gan⁃song Yin(HGY)on the glomer⁃ulus of diabetic nephropathy(DN)in rats.Methods Ten rats were randomized into the normal control group(NC).The early DN models of SD rats were reproduced by high fat feed combined with streptozotocin.The rat DN models were randomly divided into DN model groups(DN),Glenn sinensis group(DN+Gli,10 mg/kg),spikenard drink high dose group(DN+HH,10 g/kg),and spikenard drinking low dose group(DN+HL,5 g/kg),10 rats in each group.MRNA expression of miR⁃192,the target genes of Zeb2 and TGF⁃β1,Smad3 and Smad7 in renal tissue was detected by real⁃time fluorescence quantitative PCR.Western blot was used to detect the protein expres⁃sion of the above genes.Results Compared with the blank control group,the mRNA levels of TGF⁃β1,Smad3 and miR⁃192 in the renal tissue of the DN model group was significantly increased(P<0.01,respectively),while the mRNA expression of Smad7,S1P1 and Zeb2 and Smad7 was significantly decreased(P<0.01,respectively).Compared with the DN model group,the mRNA levels of TGF⁃β1,miR⁃192 and Smad3 were significantly decreased(P<0.01,respectively),and the mRNA expression of Smad7,S1P1,Zeb2 and Smad7 was significantly increased after the treatment of HGY.Conclusion HGY may improve the accumulation of extracellular matrix in the kidney of DN rats by regulating miR⁃192⁃mediated TGF⁃β1/Smads signaling pathway,contributing to protecting the kid⁃ney of early DN rats.
作者
袁玲
李嘉欣
鲁玉梅
南一
YUAN Ling;LI Jiaxin;LU Yumei;NAN Yi(Department of Pharmacology and Toxicology,School of Pharmacy,Ningxia Medical University,Yinchuan 750004,China;Ningxia Medical University to build the key Laboratory of the Ministry of Education with the Ministry of Modern Medicine,Yinchuan 750004,China)
出处
《实用医学杂志》
CAS
北大核心
2021年第2期164-169,共6页
The Journal of Practical Medicine
基金
国家自然科学基金项目(编号:81573695,81674096)
教育部“春晖计划”合作项目(编号:Z2016065)。
