摘要
采用气相色谱-质谱联用技术(GC-MS)对甘松的水蒸气蒸馏提取物、水提取物、石油醚提取物、95%乙醇提取物、乙醇提取物石油醚萃取相、乙醇提取物氯仿萃取相、乙醇提取物乙酸乙酯萃取相、乙醇提取物正丁醇萃取相、乙醇提取物剩余萃取相中所含挥发性物质进行对比分析,并考察其对DPPH·、ABTS·^(+)、超氧阴离子自由基的清除活性和对Fe^(3+)的还原能力(FRAP值)。结果表明,甘松不同提取物中共鉴别出109种化学成分;乙醇提取物正丁醇萃取相对ABTS·^(+)自由基的清除活性最强,乙醇提取物乙酸乙酯萃取相对DPPH·、超氧阴离子自由基的清除活性和对Fe^(3+)的还原能力最强。本研究可为甘松挥发性成分的临床应用提供依据。
Gas chromatography-mass spectrometry(GC-MS)was used to analyze the steam distillation extract,water extract,petroleum ether extract,95%ethanol extract,and different extractive phases from ethanol extract by different solvents such as petroleum ether,chloroform,ethyl acetate and n-butanol of Rhizoma Nardostachyis.Comparative analysis of the volatile substances contained in different extracts and extractive phases from ethanol extract were carried out,and the scavenging ability on DPPH·,ABTS·^(+),superoxide anion radicals and Fe^(3+)reduction ability(FRAP value)were investigated.The results showed that a total of 109 chemical components were identified in different extracts of Rhizoma Nardostachyis;the n-butanol extractive phase from ethanol extract present the strongest scavenging ability on ABTS·^(+),and the ethyl acetate extractive phase from ethanol extract present the strongest scavenging ability on DPPH·and superoxide anion free radicals,and the strongest reduction ability on Fe^(3+).This study can provide a basis for further research and clinical application of the volatile components of Rhizoma Nardostachyis.
作者
张小荣
何海
赵沙沙
马艳珠
崔治家
邵晶
ZHANG Xiaorong;HE Hai;ZHAO Shasha;MA Yanzhu;CUI Zhijia;SHAO Jing(College of Pharmacy,Gansu University of Chinese Medicine,Lanzhou Gansu 730000,China;Northwest Collaborative Innovation Center of Chinese and Tibetan Medicine,Lanzhou Gansu 730000,China;Provincial Key Laboratory of Chinese(Tibetan)Medicine Chemistry and Quality Research of Higher Education Institutions in Gansu Province,Lanzhou Gansu 730000,China;Key Laboratory of Pharmacology and Toxicology of Traditional Chinese Medicine in Gansu Province,Gansu University of Chinese Medicine,Lanzhou Gansu 730000,China)
出处
《中兽医医药杂志》
CAS
2023年第1期26-33,共8页
Journal of Traditional Chinese Veterinary Medicine
基金
甘肃省教育厅双一流重大科研项目(GSSYLXM-05)
中医药公共卫生服务补助专项子课题(2305191901)
甘肃中医药大学科研与创新基金项目重点项目(2020KCZD-2)。
关键词
甘松
不同提取物
GC-MS
抗氧化活性
Rhizoma Nardostachyis
different extracts
GC-MS
antioxidant activity
