摘要
目的参加编号为NIFDC-PT-220的能力验证,考核从乳粉中检出沙门氏菌及血清分型能力。方法参照国标方法对中国食品药品检定研究院提供的随机乳粉样品(CODE140、CODE121)进行沙门氏菌的检验,并将荧光酶联免疫法和荧光定量PCR技术作为辅助方法。对分离出的疑似菌进行生化鉴定,并且采用全自动微生物鉴定系统进行全面鉴定。结果编号CODE140血清分型为O:4,5,12,H:i;1,2被确定为鼠伤寒沙门氏菌。编号CODE121未检出。结论荧光酶联免疫法和荧光定量PCR技术缩短了检测时间,特异性和敏感性好,与国标方法配合可确保实验结果的准确性。
Objective To participate in the ability verification of NIFDC-PT-220 to test the ability of detecting Salmonella from milk powder and serotyping.Methods According to the national standard method,the random milk powder samples(CODE140,CODE121)provided by the China Food and Drug Inspection Institute were tested for Salmonella,and the fluorescent enzyme-linked immunoassay and fluorescent quantitative PCR technology were used as auxiliary methods.Biochemical identification of suspected isolated bacteria was performed,and the automatic microbial identification system was used for the overall identification.Results Salmonella typhimurium was detected in CODE 140,the serotype was O:4,5,12,H:i;1,2.Salmonella spp.was not detected in CODE 121.Conclusion Fluorescence enzyme-linked immunoassay and fluorescence quantitative PCR shortened the detection time and showed good specificity and sensitivity.Cooperating with the national standard method can ensure the accuracy of the experimental results.
作者
沈锐
杨荣荣
王梅
SHEN Rui;YANG Rong-Rong;Wang Mei(Chongqing Yongchuan Institute for Food and Drug Control,Chongqing 402160,China)
出处
《食品安全质量检测学报》
CAS
2020年第13期4514-4520,共7页
Journal of Food Safety and Quality
关键词
沙门氏菌
能力验证
分离
鉴定
血清分型
Salmonella
proficiency testing
isolation
identification
serotyping
