摘要
目的:研究芯芭醇提物和不同极性部位对常见致病菌的抑制作用,初步判断芯芭的抑菌活性部分.方法:采用琼脂扩散法分别测定芯芭醇提物和不同极性部分对测试菌株(金黄色葡萄球菌、表皮葡萄球菌、枯草芽孢杆菌、铜绿假单胞菌、肺炎克雷伯菌、大肠埃希菌、沙门菌)的抑菌活性,并通过微量肉汤稀释法测定最低抑菌浓度(MIC).结果:芯芭的醇提物和四个极性部分对测试菌株均有抑菌活性,乙酸乙酯部分对测试菌株的最低抑菌浓度可达0.94 mg/mL.结论:芯芭具有广谱抑菌活性,具有抑菌活性的部分是乙酸乙酯部分.
Objective:To investigate the antimicrobial activities of Cymbaria daurica L.extracts on Common pathogenic microorganisms,and determine the antibacterial activity of Cymbaria daurica L.Methods:Agar diffusion test was used to study Cymbaria daurica L.extracts antimicrobial activities on the Gram-positive bacteria Staphylococcus epidermidis,Staphylococcus aureus and Bacillus subtilis and the Gram-negative bacteria Pseudomonas aeruginosa,Klebsiella Pneumoniae,Escherichia coli and Salmonella,then determined the minimum inhibitory concentration(MIC)by using liquid dilute method.Results:All the five fractions showed inhibitory effects on experimental bacteria,in the ethyl acetate extract,the minimum inhibitory concentration of the tested strain was 0.94 mg/mL.Conclusion:The study suggested that Cymbaria daurica L.had broad-spectrum bacteriostatic activity.The part produced the antibacterial activity may be the ethyl acetate extract.
作者
石如玉
张美英
阿古拉
龚雪
张春红
李旻辉
SHI Ru-yu;ZHANG Mei-ying;Agula;GONG Xue;ZHANG Chun-hong;LI Min-hui(Baotou Medical College,Baotou 014060,China;Inner Mongolia Autonmous Region Academy of Chinese Medicine,Hohhot 010110,China;Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement,Guangxi Botanical Garden of Medicinal Plant,Nanning 530023,China;Inner Mongolia Research Center of Characteristic Medicinal Plants Cultivation and Protection Engineering Technology,Baotou 014060,China;Inner Mongolia Key Laboratory of Characteristic Traditional Chinese Medicinal Resources Protection and utilize,Baotou 014060,China)
出处
《中国民族医药杂志》
2020年第3期22-24,共3页
Journal of Medicine and Pharmacy of Chinese Minorities
基金
国家自然科学基金项目(81760776)
内蒙古自治区自然科学基金项目(2018ZD13)。
关键词
芯芭
抑菌活性
琼脂扩散实验
最低抑菌浓度
Cymbaria daurica L.:Antimicrobial activities
Agar diffusion test
Minimum inhibitory concentration
