摘要
目的分析慢性肾脏病(chronic kidney disease,CKD)患者中成纤维细胞生长因子23(fibroblast growth factor 23,FGF23)水平与促炎性趋化因子之间的相关性。方法选取神木市医院肾脏病科2017年1月~2019年1月收治的50例CKDⅡ~Ⅴ期患者和50例老年健康体检对照组纳入研究,分析受试对象血液和尿液中的生化指标。用ELISA技术测量FGF23水平,通过Milliplex技术检测分析促炎细胞因子,并分析其相关性。结果CKD患者的肿瘤坏死因子(TNF)-α调节激活正常T-细胞表达分泌因子(RANTES)和白介素(IL)-12水平均显著升高,差异有统计学意义(t=3.965,4.087,4.956,均P<0.001)。CKD患者的FGF23水平与尿清蛋白排泄(UAE)、磷酸盐、甲状旁腺激素(PTH)和钙之间存在显著相关性,差异有统计学意义(P<0.001),与C反应蛋白(C reactive protein,CRP)和纤维蛋白原之间无显著相关性(P>0.05)。FGF23水平与RANTES和IL-12之间具有显著相关性,差异有统计学意义(P<0.001)。CKD组中IL-8,IFN-γ和TNF-α与FGF23水平无相关性(P>0.05)。CKD患者的FGF23与GFR之间存在显著负相关[r=-0.66(95%CI:-0.78~0.62),P<0.001],随着GFR水平的降低,FGF23的浓度显著增加。结论CKD患者处于炎症状态。FGF23水平与钙磷代谢、促炎性细胞因子、肾功能恶化程度相关,可作为预测病程进展的早期指标。
Objective To analyze the correlation between FGF23 levels and pro-inflammatory chemokines in patients with chronic kidney disease( CKD). Methods Fifty CKD patients with stage 2-5 and 50 geratic healthy controls were analyzed for biochemical indicators in blood and urine in the Shenmu Hospital from January 2017 to December 2019. FGF23 levels were measured by ELISA,Proinflammatory cytokines were analyzed by Milliplex technique and the correlation was analyzed. Results The levels of tumor necrosis factor( TNF)-α,RANTES and interleukin( IL)-12 were significantly higher in CKD patients with statistically significant differences( t = 3. 965,4. 087,4. 956,all P < 0. 001). There was a significant correlation between FGF23 leves and urinary albumin excretion,phosphate,PTH,and calcium in patients with CKD and the difference was statistically significant( P < 0. 001),but no significant correlation with C reactive protein( CRP) and fibrinogen( P > 0. 05),and there was a significant correlation between FGF23 levels and the cytokines RANTES and IL-12 with statistically significant differences( P < 0. 001). In the CKD group,IL-8,IFN-γ and TNF-α were not related to FGF23 levels( P > 0. 05). There was a significantly negative correlation between FGF23 and GFR in CKD patients [r =-0. 66( 95% CI:-0. 78 to 0. 62),P< 0. 001],the concentration of FGF23 increased significantly as the level of GFR decreased. Conclusion CKD patients are in an inflammatory state. The level of FGF23 is associated with calcium and phosphorus metabolism,pro-inflammatory cytokines,and deterioration of renal function,and can be used as an early indicator of disease progression.
作者
杨雄
尹爱萍
YANG Xiong;YIN Ai-ping(Medical College of Xi'an Jiaotong University,Xi'an 710061,China;Department of Nephrology,Shenmu Hospital,Shaanxi Shenmu 719300,China;the First Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710061,China)
出处
《现代检验医学杂志》
CAS
2020年第1期25-28,共4页
Journal of Modern Laboratory Medicine
