摘要
啤酒腐败菌会影响啤酒的质量和安全,因此,啤酒生产过程中腐败菌的检测尤为重要。传统的培养基检测方法简单、易操作且准确性强、但耗时较长。随着现代啤酒工业和分子生物学技术的不断发展,快速、灵敏的检测技术层出不穷,如改进后的培养基法、生化发光检测法、基于PCR的检测方法、基于核酸杂交的检测方法和基质辅助激光解吸电离飞行时间质谱等。本文从方法、原理、优缺点等多个方面总结和阐述啤酒腐败菌的检测方法,以期为啤酒生产过程中高效、准确的检测腐败菌提供参考。
Beer spoilage microorganisms can affect the quality of beer and also cause some safety problems.Therefore,the detection of spoilage microorganisms in brewing process is quite necessary and important.The traditional cultivation method is simple,easy and precise,but it is very time-consuming.With the development of the brewing industry and the molecular biology technology,a number of detection technologies,which are effective and sensitive,have emerged.These technologies include the modified cultivation methods,bioluminescence as well as chemiluminescence method,PCR-based methods,nucleic acid hybridization-based method and maldi-tof mass spectrometry method.In this article,the progresses and the principles on the above mentioned methods were summarized,and their advantages as well as the limitations were disscussed.This review will provide some useful information for the detection of beer spoilage microorganisms in brewing process.
作者
余偲
张宝善
李娜
刘巧
李爱萍
孙聿珩
毛伟文
赵育
YU Si;ZHANG Bao-shan;LI Na;LIU Qiao;LI Ai-ping;SUN Yu-heng;MAO Wei-wen;ZHAO Yu(College of Food Engineering and Nutritional Science,Shaanxi Normal University,Xi5 an 710119,China)
出处
《食品工业科技》
CAS
北大核心
2020年第2期324-329,共6页
Science and Technology of Food Industry
基金
陕西师范大学中央高校基本科研业务费项目(GK201803067)
2019年度陕西省科技计划项目-科技成果转移与推广计划(2019CGXNG-036)
2019年度陕西省科技计划项目-农业领域(2019NY-132)
2017年陕西省重点产业创新链项目(2017ZDCXL-NY-01-02)
关键词
啤酒腐败菌
检测方法
培养基法
PCR检测法
核酸杂交
spoilage bacteria
detection methods
cultivation methods
PCR detection methods
nucleic acid hybridization
