摘要
建立一种快速准确的柱前衍生-超高效液相色谱法,同时测定黄酒中17种游离氨基酸含量。黄酒样以6-氨基喹啉-N-羟基琥珀酰亚胺基氨基甲酸酯(AQC)衍生化处理,以流速为0.7 mL/min经Waters AccQ·TagTM Ultra C18(1.7μm,2.1×100 mm)的色谱柱梯度洗脱,利用二极管阵列检测器,在260 nm波长处检测。结果显示,17种游离氨基酸浓度在25~400μmol/L范围内,峰面积与浓度之间具有良好的线性关系(R2≥0.9993),回收率在94.21%~104.82%。该方法可在11 min内将17种游离氨基酸完全分离,具有准确、快速、重复性好的特点,可用于黄酒中游离氨基酸含量的测定。
A rapid method of UPLC coupled with pre-column derivatization has been developed for simultaneous determination of 17 kinds of free amino acids in Huangjiu.Wine samples were derived with 6-aminoquinolyl-N-hydroxysuccinimidylcarbamate(AQC)as derivatization agent,then separated on a Waters AccQ·TagTM Ultra C18 column(1.7μm,2.1×100 mm)at a flow rate of 0.7 mL/min,and finally detected by using PDA detector at the wavelength of 260 nm(UV).The results showed that,the concentration of the 17 amino acids had good correlation(R2≥0.9993)with peak areas in the range of 25μmol/L to 400μmol/L,and the spike recoveries were between 94.21%to 104.82%.All the 17 free amino acids could be separated completely within 11 min by use of this method.The method was featured by accurate and rapid determination with good repeatability.
作者
田翔
王君杰
秦慧彬
乔治军
TIAN Xiang;WANG Junjie;QIN Huibin;QIAO Zhijun(Institute of Crop Variety Resources,Shanxi Academy of Agricultural Sciences,Taiyuan,Shanxi 030031;Key Lab of Crop Gene Resources and Germplasm Enhancement on Loess Plateau,Ministry of Agriculture,Taiyuan,Shanxi 030031;Shanxi Key Lab of Genetic Resources and Genetic Improvement of Minor Crops,Taiyuan,Shanxi 030031,China)
出处
《酿酒科技》
2019年第11期74-78,82,共6页
Liquor-Making Science & Technology
基金
山西省农业科学院特色农业技术攻关项目(YGG17058)
国家现代农业产业技术体系项目(CARS-06-13.5-A16)
关键词
黄酒
超高效液相色谱法
氨基酸
Huangjiu
ultra performance liquid chromatography(UPLC)
amino acids
