摘要
为了证实防风抽薹后品质下降,该研究利用高效液相法(HPLC)对防风药材皮部和木部4个色原酮类成分(升麻素苷、5-O-甲基维斯阿米醇苷、升麻素、亥矛酚苷)的含量进行分析,并建立了其化学指纹图谱,标定了其中的12个共有峰,相似度分析结果发现各批次样品之间的相似度在0. 115~0. 995,说明防风药材的皮部和木部具有一定的差异性。在此基础上,以4个色原酮类成分的含量以及是否符合药典标准作为原变量进行系统聚类分析、主成分分析和正交偏最小二乘判别分析,结果表明4个成分在防风皮部的含量远高于木部,所检测的4个成分能够较好的区分防风皮部和木部。研究结果以现代分析方法揭示了防风抽薹后根部木化,品质下降。
In order to confirm the tradition that bolting Saposhnikoviae Radix could not be used as medicine,the content of four chromone components in the cortex and wood of Saposhnikoviae Radix was analyzed by high performance liquid chromatography( HPLC),and the chemical fingerprints were established,12 common peaks were calibrated. The similarity analysis found that the similarity between batches was 0. 115-0. 995,it indicates that the cortex and wood of Saposhnikoviae Radix have certain differences. On this basis,systematic clustering analysis,principal component analysis and orthogonal partial least squares discriminant analysis were carried out with the content of four chromone components and whether they met the pharmacopoeia criteria as the original variables. The results showed that the content of the four components in the cortex of Saposhnikoviae Radix was much higher than that in the wood,and the four components detected were able to distinguish the cortex and the wood of Saposhnikoviae Radix. The results of the study reveal the tradition that bolting Saposhnikoviae Radix should not be used as medicine dut to decreased quality.
作者
张丹
木盼盼
郭梅
王继伟
郑开颜
侯芳洁
郭龙
郑玉光
ZHANG Dan;MU Pan-pan;GUO Mei;Wang Ji-wei;ZHENG Kai-yan;HOU Fang-jie;GUO Long;ZHENG Yu-guang(College of Pharmacy,Hebei University of Chinese Medicine,Shijiazliuang 050200,China;Chengde Petroleum College,Chengdu 067000,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2019年第18期3948-3953,共6页
China Journal of Chinese Materia Medica
基金
河北省自然科学基金青年项目(H2018423029)
河北省高等学校科学技术研究项目(QN2016103)
中央财政公共卫生专项(Z135080000022)
全国中药炮制技术传承基地项目(冀中医药[2017]37号)
河北省第二期现代农业产业技术体系创新团队项目(HBCT2018060205)
河北省中医药管理局科研计划项目(2015079)
