摘要
为创制天麻种子材料用于开展天麻有性繁殖分子机制研究奠定基础。该文对红天麻和乌天麻在变温(室温)和恒温(22℃)下抽薹后的花、蒴果、种子、种胚等13个性状进行分析,结果发现,恒温条件可以延长天麻抽薹持续时间,且能增加花朵数量,而天麻品种仅影响抽薹持续时间,对花朵数量无影响,并且红天麻比乌天麻更易抽薹;品种是影响天麻蒴果裂果主要的因素,温度是影响蒴果数和蒴果直径主要的因素,而恒温比室温更利于天麻蒴果的形成;天麻品种在种子的外观上无明显差异,而恒温条件下培育的天麻种子种胚比室温条件下培育的种子更加饱满;温度和品种对天麻种子外观的影响较小,而对种子的种胚的影响较大,恒温培育更利于天麻种子种胚的形成,红天麻比乌天麻种子更易形成种胚。该研究表明,乌天麻和红天麻种子发育可直接选择22℃恒温进行抽薹生产。
The study is aimed to create seed materials and dissect the molecular mechanism of sexual propagation of Gastrodia elata.In this research,thirteen characteristics of flowers,flower stem,fruits,seeds and embryo of G.elata f.glauca and G.elata f.elata after bolting at room temperature(RT)and constant temperature(CT,22℃)were determined.It was found that the constant temperature condition could prolong the bolting duration of G.elata and increased the number of flowers,while the variety of G.elata only affected the bolting duration,but had no effect on the number of flowers,and the G.elata f.elata was more likely to bolting than the G.elata f.glauca.The variety of G.elata was the main factor affecting the time of dehiscent fruit of G.elata,the temperature was the main factor affecting the fruits number and fruits diameter,and the constant temperature was more conducive to the fruits shape of G.elata than the room temperature.There was no significant difference in seed phenotype of G.elata varieties,but the seed embryo of G.elata seeds cultivated at constant temperature was fuller than that of G.elata cultivated at room temperature,and temperature had less influence on the seed phenotype of G.elata.But it was interesting to find that temperature and varieties had greater influence on the seed embryo of G.elata,constant temperature cultivation was more conducive to the formation of seed embryo of G.elata,and more the seed embryo of G.elata f.elata was easier to form than the seed embryo of G.elata f.glauca.However,the development of seeds and embryos of G.elata was significantly affected,and the development of seeds and embryos of G.elata f.glauca was more sensitive to temperature than G.elata f.elata.The research suggested that it is advisable for G.elata to produce seed materials by bolting at constant temperature(22℃).
作者
袁青松
王绘
江维克
欧小宏
徐娇
王小爱
汪露
欧跃云
周涛
YUAN Qing-song;WANG Hui;JIANG Wei-ke;OU Xiao-hong;XU Jiao;WANG Xiao-ai;WANG Lu;OU Yue-yun;ZHOU Tao(Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China;Guizhou Tiancheng Agricultural Science and Technology Development Co.,Ltd.,Dafang 551600,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2020年第3期485-490,共6页
China Journal of Chinese Materia Medica
基金
中央本级重大增减支项目(2060302)
国家重点研发计划中医药现代化研究专项(2017YFC1700703)
国家自然科学基金项目(81960694)
贵州省科技创新人才团队项目(黔科合平台人才[2019]5611)
贵州省高层次创新型人才项目(黔科合平台人才[2018]5638)
贵州省教育厅创新群体重大研究项目(黔教合KY字[2018]022)
贵州省科技计划项目(黔科合支撑[2018]2764)
贵州中医药大学博士启动基金项目([2019]55).
关键词
天麻
种子发育
温度
抽薹
Gastrodia elata
seed development
temperature
bolting
