摘要
为了建立乌梅颗粒的质量标准,采用薄层色谱法(TLC)对乌梅、黄连进行鉴别;用高效液相色谱法测定乌梅颗粒中枸橼酸的含量。色谱条件为SilgreenC18(4.6mm×250mm,5μm)色谱柱;流动相为乙腈-0.5%磷酸二氢铵溶液(3∶97)(用磷酸调节pH值至3.0);检测波长为210nm;流速为0.6mL/min;柱温为室温。研究建立了乌梅、黄连的薄层色谱定性鉴别的方法;建立了HPLC测定乌梅颗粒中枸橼酸含量的方法。建立的乌梅颗粒鉴别及枸橼酸含量测定方法简单、易于操作、准确度高、重现性好,可用于乌梅颗粒的质量控制。
To establish the quality standard of Wumei Granules. Thin layer chromatography (TLC) was used to identify the black plum and coptis chinensis. The content of citric acid in wumei granules was determined by HPLC. The chromatographic condition was Silgreen C18 (4.6 mm×250 mm, 5μm) column. The mobile phase was acetonitrile-0.5% ammonium dihydrogen phosphate solution (3 ∶ 97)(pH value adjusted by phosphoric acid to 3.0). The detection wavelength was 210 nm. The flow rate was 0.6 mL/min. The column temperature is room temperature. A TLC method for the qualitative identification of fructus mume and rhizoma coptidis was established. HPLC method for determination of citric acid in wumei gran ules was established. The method is simple, easy to operate, accurate and reproducible. It can be used for quality control of Wumei Granules.
作者
张远达
陈志强
尚静雪
蔡小利
战文
张鹏举
付丽云
李海华
ZHANG Yuan-da;CHEN Zhi-qiang;SHANG Jing-xue;CAI Xiao-li;ZHAN Wen;ZHANG Peng-ju;FU Li-yun;LI Hai-hua(Inner Mongolia huatian pharmaceutical co., LTD., chifeng city, Inner Mongolia 024070,China)
出处
《中国兽药杂志》
2019年第10期27-32,共6页
Chinese Journal of Veterinary Drug
基金
2018年内蒙古科技重大项目
关键词
乌梅颗粒
枸橼酸
薄层色谱法
高效液相色谱法
Wumei Granules
Citrate
thin layer chromatography
high performance liquid chromatography
