摘要
目的建立高效液相色谱法测定便秘糖衣丸中比沙可啶、番泻苷A和番泻苷B的含量。方法采用Alltima C18色谱柱(150 mm×4.6 mm,5μm),乙腈-30 mmol·L^-1磷酸二氢铵溶液为流动相,流速为1.0m L·min^-1,比沙可啶的检测波长为265 nm,番泻苷A和番泻苷B的检测波长为340 nm。结果比沙可啶、番泻苷A与番泻苷B的线性范围分别为26.23~419.68μg·mL^-1、9.72~194.44μg·m L^-1、9.54~190.72μg·m L^-1,相关系数分别为0.9998、0.9997、0.9993,上述3种化学成分平均加样回收率分别为98.6%、97.5%、97.2%,RSD均小于3%。结论该方法可用于测定便秘糖衣丸中比沙可啶、番泻苷A和番泻苷B的含量。
Objective To establish a high performance liquid chromatography(HPLC) method to determine the content of bisacodyl, sennoside A and sennoside B in Surulac-S. Methods An Alltima C18 column(150 mm×4.6 mm, 5 μm) was used for the separation with mobile phase of acetonitrile-30 mmol·L^-1 ammonium dihydrogen phosphate solution at 1.0 mL·min^-1. The detection wavelength was 265 nm for bisacodyl while 340 nm for both sennoside A and sennoside B. Results The linear ranges of bisacodyl, sennoside A and sennoside B were 26.23-419.68 μg·mL^-1, 9.72^-194.44 μg·mL^-1, and 9.54^-190.72 μg·mL^-1, with correlation coefficients of 0.9998, 0.9997 and 0.9993, respectively. The average recoveries of the 3 components were 98.6%, 97.5% and 97.2% with RSDs all below 3%. Conclusion The method is suitable for the determination of bisacodyl, sennoside A and sennoside B in Surulac-S.
作者
徐文峰
金鹏飞
徐硕
姜文清
吴学军
邝咏梅
徐巧玲
XU Wen-feng;JIN Peng-fei;XU Shuo;JIANG Wen-qing;WU Xue-jun;KUANG Yong-mei;XU Qiaoling(Department of Pharmacy,National Center of Gerontology,Beijing Hospital,Key Laboratory of Assessment of Clinical Drugs Risk and Individual Application,Bejing 100730;Department of Pharmacy,The 305th Hospital of the PLA of China,Bejing 100017)
出处
《中南药学》
CAS
2019年第9期1525-1528,共4页
Central South Pharmacy
基金
国家自然科学基金项目(No.81803715)
北京医院科技新星项目(No.BJ-2016-039)
