摘要
采用高效液相色谱法测定杨梅中的有机酸,流动相为0.02 mol/L的NaH2PO4,检测波长为210 nm。柠檬酸、苹果酸、酒石酸、乳酸的检出限分别为5、3、1、10 mg/L,测定结果的相对标准偏差分别为0.2%、0.3%、0.1%、2.4%,线性范围分别为0.01-0.7 g/L、0.05-0.7 g/L、0.1-0.7 g/L、0.1-0.7 g/L,线性相关系数分别为0.9999、0.9997、0.9911、0.9944。实验结果表明,杨梅中含有柠檬酸、苹果酸、乳酸、酒石酸、草酸,其中以柠檬酸含量最高。
Organic acid in redbayberry was determined by high performance liquid chromatography.The mobile phase was 0.02 mol / L NaH2PO4,and detection dewavelength was 210 nm.The detection limits of citric acid,malic acid,lactic acid and tartaric acid were 5,3,1,10 mg/L respectively.The relative standard deviations were 0.2%,0.3%,0.1%,2.4% respectively,and the linear ranges were 0.01-0.7 g/L,0.05-0.7 g/L,0.1-0.7 g/L,0.1-0.7 g/L with the linear correlation coefficients of 0.999 9,0.999 7,0.991 1,0.994 4 respectively. The result showed that redbayberry contained citric acid, malic acid, lactic acid, tartaric acid and oxalic acid. The content of citric in redbayberry acid was the highest.
出处
《化学分析计量》
CAS
2009年第3期38-40,共3页
Chemical Analysis And Meterage
关键词
高效液相色谱
杨梅
有机酸
high performance liquid chromatography
redbayberry
organic acid
