摘要
目的:研究芪蛭通络胶囊及其9味活血化瘀药拆方对大鼠脑缺血再灌注损伤的保护作用,为通过拆方的方法研究芪蛭通络胶囊活性成分奠定基础。方法:将60只SD大鼠随机分为假手术组(0.5%羧甲基纤维素钠)、模型组(0.5%羧甲基纤维素钠)、芪蛭通络胶囊缺活血化瘀药味组[0.389 g/(kg·d)]、芪蛭通络胶囊活血化瘀药味组[0.253 g/(kg·d)]和芪蛭通络胶囊成品组[0.500 g/(kg·d)],每组12只;各组大鼠均灌胃给药,每天1次,连续14 d;末次给药2 h后,除假手术组外,其余各组大鼠均采用线栓法复制脑缺血再灌注损伤模型。缺血2 h再灌注24 h后,依据Bederson评分法对各组大鼠进行神经功能评分,氯化三苯基四氮唑(TTC)染色法计算其脑梗死面积,生化法测定大鼠脑组织中一氧化氮(NO)、丙二醛(MDA)、乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)的含量,酶联免疫吸附法检测大鼠脑组织中白细胞介素1β(IL-1β)、肿瘤坏死因子α(TNF-α)的含量。结果:与假手术组比较,模型组大鼠的神经功能评分显著升高(P<0.05),脑梗死面积和脑组织中NO、MDA、LDH、IL-1β、TNF-α含量均显著增加(P<0.05),脑组织中SOD含量显著减少(P<0.05)。与模型组比较,各给药组大鼠神经功能评分均显著降低(P<0.05),脑梗死面积和脑组织中NO、MDA、LDH、IL-1β、TNF-α含量均显著减少(P<0.05),脑组织中SOD含量显著增加(P<0.05)。与芪蛭通络胶囊成品组比较,芪蛭通络胶囊活血化瘀药味组和芪蛭通络胶囊缺活血化瘀药味组大鼠脑梗死面积和脑组织中NO、MDA、LDH、IL-1β、TNF-α含量均显著增加(P<0.05),SOD含量均显著减少(P<0.05)。结论:芪蛭通络胶囊的全方及其9味活血化瘀药(或不含9味活血化瘀药)的拆方对大鼠脑缺血再灌注损伤均具有一定的保护作用,活血化瘀药味拆方的保护作用虽不及全方,但全方缺活血化瘀药味后其保护作用也明显下降,表明活血化瘀药在全方发挥保
OBJECTIVE:To study the protective effects of Qizhi tongluo capsules and their 9 ingredients decomposed recipes for promoting blood circulation and removing blood stasis(PBCRBS)on cerebral ischemia-reperfusion injury model rats,and to lay a foundation for studying the active components of Qizhi tongluo capsules through decomposed recipes. METHODS:Totally 60 SD rats were randomly divided into sham operation group(0.5% CMC-Na),model group(0.5% CMC-Na),Qizhi tongluo capsules without the ingredients for PBCRBS group [0.389 g/(kg·d)],the ingredients of Qizhi tongluo capsules for PBCRBS group[0.253 g/(kg·d)] and Qizhi tongluo capsules group [0.500 g/(kg·d)],with 12 rats in each group. The rats were given relevant medicine intragastrically,once a day,for consecutive 14 d. 2 h after last medication,except for sham operation group,cerebral ischemia-reperfusion injury model was induced by suture method in each group. The neurological function score was measured according to Bederson score 2 h after ischemia and 24 h after reperfusion. The area of cerebral infarction was calculated by TTC staining. The contents of NO,MDA,LDH and SOD in cerebral tissue of rats were measured by biochemical method. The contents of IL-1β and TNF-α in cerebral tissue of rats were detected by ELISA. RESULTS:Compared with sham operation group,the neurological function score,the infarct area and the contents of NO,MDA,LDH,IL-1β and TNF-α in cerebral tissue were significantly increased in model group(P<0.05). The content of SOD in cerebral tissue were decreased significantly(P<0.05).Compared with model group,the neurological function score of rats was decreased significantly in each administration group(P<0.05);the area of cerebral infarction,the contents of NO,MDA,LDH,IL-1β and TNF-α in cerebral tissue were decreased significantly(P<0.05),while the content of SOD in cerebral tissue was increased significantly(P<0.05). Compared with Qizhi tongluo capsules group,the area of cerebral infarction,the contents of NO,MDA,LDH,IL-1β and TNF-α in c
作者
武晓伟
刘聪
李佳
李祥
倪艳
WU Xiaowei;LIU Cong;LI Jia;LI Xiang;NI Yan(Shanxi Provincial Academy of TCM,Taiyuan 030012,China)
出处
《中国药房》
CAS
北大核心
2019年第19期2674-2678,共5页
China Pharmacy
基金
山西省科技攻关项目(No.2016ZD0402)
关键词
芪蛭通络胶囊
拆方
活血化瘀
脑缺血再灌注损伤
大鼠
Qizhi tongluo capsules
Decomposed recipes
Promoting blood circulation and removing blood stasis
Cerebral ischemia reperfusion
Rat
