摘要
目的探究葛花解酲汤及其拆方组对急性酒精性中毒小鼠模型的防醉解酒作用。方法将小鼠随机分为空白组、醉酒模型组、葛花解酲汤全方组、减理气组、减利水组、减健脾组、减温中组、减葛花组。将生理盐水灌入空白组小鼠胃中,给药各组按葛花解酲汤全方组0.24 mL/20 g、减理气组0.14 mL/20 g、减利水组0.2 mL/20 g、减健脾组0.18 mL/20 g、减温中组0.23 mL/20 g、减葛花组0.2 mL/20 g灌胃给予药物,醉酒模型组给予0.2 mL/20 g生理盐水。30 min后,用56%乙醇,按照0.2 mL/20 g的剂量给予醉酒模型组和给药组小鼠进行灌胃。以急性酒精中毒小鼠翻正反射时间、自主活动次数、平衡失误次数、逃避潜伏期时间、对目标象限跨越平台次数、血中乙醇浓度作为防醉解酒作用的衡量指标。结果与醉酒模型组相比,葛花解酲汤全方组、减温中组醉酒潜伏期明显的延长,全方组的醒酒时间明显缩短;与空白组相比,醉酒模型组的自主活动次数明显增高,与醉酒模型组进行对比,葛花解酲汤全方组、减温中组自主活动次数明显降低,减理气组、减利水组、减健脾组、减葛花组的自主活动次数无明显降低;与空白组相比,醉酒模型组的平衡失误次数明显偏高,与醉酒模型组相比,葛花解酲汤全方组、减健脾组、减温中组的平衡失误次数显著降低;与空白组相比,醉酒模型组的逃避潜伏期时间明显偏长,与醉酒模型组相比,葛花解酲汤全方组、减利水组、减温中组的逃避潜伏期显著减少;与空白组相比,醉酒模型组的跨越次数明显减少,与醉酒模型组相比,葛花解酲汤全方组的跨越次数显著增高;与醉酒模型组相比,葛花解酲汤全方组的乙醇浓度有明显的下降,其余给药组改变不明显。结论葛花解酲汤有明显的防醉解酒的作用,拆方组中,葛花组、理气组、健脾组作用最强。
Objective:To investigate the relieving alcoholism and anti-temulence of Gehua Jiecheng decoction and its decomposed group on acute alcoholism mice model.Methods:Mice were randomly divided into the blank group,the drunken model group,the Gehua Jiecheng decoction group,the reduced liqi medicine group,the reduced Lishui medicine Group,the reduced Jianpi medicine group,the reduced wenzhong medicine group,and the reduced gehua group.The blank group was gavaged with normal saline.The drug group mice were gavaged with the Gehua Jiecheng decoction 0.24 mL/20 g,the reduced liqi medicine 0.14 mL/20 g,the reduced Lishui medicine 0.2 mL/20 g,the reduced Jianpi medicine 0.18 mL/20 g,the reduced wenzhong medicine 0.23 mL/20 g,and the reduced gehua 0.2 mL/20 g.The drunken model group mice were gavaged with normal saline 0.2 mL/20 g.After 30 minutes,the model group and the mice in the administration group were given 56% ethanol 0.2 mL/20 g for gavage.In the acute alcoholism mice,the time of righting reflex,the number of independent activities,the number of balance errors,the time to escape the latency period,the number of times across the target quadrant,and the concentration of ethanol in the blood were used as indicators of relieving alcoholism and anti-temulence.Results:Compared with the model group,the drunk incubation period of the whole group and the reduced wenzhong medicine group was significantly prolonged,and the soberness time of the whole group was significantly shortened.Compared with the blank group,the number of independent activities of the model group is significantly higher;Compared with the model group,the number of independent activities in the whole group and the reduced wenzhong medicine group was significantly decreased.The number of independent activities of the reduced liqi medicine group,the reduced lishui group,the reduced jianpi group and the reduced Gehua group was not significantly decreased;Compared with the blank group,the number of balance errors of the model group is significantly higher,Compare
作者
胡晓阳
赵启腾
朴圣爱
HU Xiaoyang;ZHAO Qiteng;PIAO Shengai(Jixi People's Hospital Medical Group,Jixi 158100,Heilongjiang,China;Heilongjiang University of Chinese Medicine,Harbin 150036,Heilongjiang,China;The First Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150036,Heilongjiang,China)
出处
《辽宁中医药大学学报》
CAS
2020年第1期18-21,共4页
Journal of Liaoning University of Traditional Chinese Medicine
基金
国家自然科学基金青年科学基金(81202638)
黑龙江省政府博士后资助项目(LBH-Z7210).
关键词
葛花解酲汤
拆方研究
解酒作用
小鼠
Gehua Jiecheng decoction
disintegrationstudy
anti-temulence effect
mouse
