摘要
目的在局灶性脑缺血再灌注模型制备中对线栓法进行改良,以促进线栓顺利进出,提高2,3,5-氯化三苯基四氮唑(TTC)染色质量。方法取SPF级雄性SD大鼠60只,随机分为A组(常规栓线组)、B组(栓线加长组)和C组(栓线加长和改良TTC染色组),每组20只,制备局灶性脑缺血再灌注模型。术后比较分析三组大鼠的死亡率、栓线回缩数量、神经功能评分、拔栓线时所需时间、脑组织病理学切片及TTC染色结果。结果 B、C组大鼠栓线回缩至切口发生率(均为0)低于A组[40.0%(8/20)],再灌注耗时[(1.09±1.90)、(1.13±0.31)min]较A组[(5.57±1.90)min]明显缩短,差异均有统计学意义(P<0.05)。术后12 h,B、C组神经功能缺损评分[(2.32±0.63)、(2.35±0.46)分]与A组[(2.42±0.50)分]比较,差异无统计学意义(P>0.05)。苏木精-伊红染色结果显示,B、C组病理组织学改变与A组相似。TTC染色结果显示,A、B两组染色后颜色分明,对比明显,C组即便用锡箔纸进行避光处理,避光染色效果仍不理想。结论延长栓线长度能简化再灌注退栓线时的操作,采用棕色安瓿瓶进行TTC染色能提高染色质量。
Objective To improve the suture-occluded method in preparing the focal cerebral ischemia reperfusion model for promoting the smooth entry and exit of thread embolism and increasing 2,3,5-triphenyl tetrazolium chloride （TTC） staining quality. Methods 60 SPF grade male SD rats were selected and randomly divided into the group A （routine suture-occluded group）, B （lengthened embolic thread group ） and C （lengthened embolic thread plus improved TTC staining group）, 20 cases in each group. The focal cerebral ischemia reperfusion model was prepared. The postoperative mortality rate, number of thread embolism retraction, neural function score, needing time for pulling out thread embolism, brain tissue pathological section and TTC staining results were compared among 3 groups. Results The occurrence rate of thread embolism retraction to incision in ythe group B and C was 0,which was lower than 40.0% （8/20） in the group A; the elapsed time for reperfusion in the group B and C was（1.09±1.90） and（ 1.13±0.31 ）rain respectively,which was significantly shortened compared with （5.57±1.90）min in the group A, the differences were statistically significant（P〈0.05 ）. The neurological impairment scores at postoperative 12 h in the group B and C were （2.32±0.63）points and （2.35±0.46）points respectively, which showed no statistical difference compared with （2.42±0.50） points in the group A （P〉0.05）. The HE staining results showed that the histopathological change in the group B and C was similar to that in the group A. After TTC staining, the color in the group A and B was clear with obvious contrast, but the effect for avoiding light and staining in the group C was still undesirable,even though conducting the avoid light processing by using the silver paper. Conclusion Extending the embolie thread length may simplify the operating for withdrawing the embolic thread in reper- fusion and adopting the brown ampoules for conducting staining can improve the staining quality.
出处
《现代医药卫生》
2016年第2期161-163,166,共4页
Journal of Modern Medicine & Health
基金
贵州省科学技术基金资助项目(黔科合JZ字[2014]2015号)
