摘要
【目的】观察电针联合大黄、水牛角煎液对脑梗死大鼠的治疗机制。【方法】将40只大鼠随机分为5组,即假手术组、模型组、电针组、中药组、针药组,每组8只。除假手术组,其他各组大鼠采用线栓法建立脑梗死模型,而假手术组分离动脉后不结扎。建模成功后,电针组针刺百会、足三里、水沟穴,中药组灌胃5 g/kg大黄、水牛角煎剂,针药组联合给予电针组与中药组的干预方法,模型组与假手术组灌胃等体积的生理盐水,治疗14 d。干预第1、7、14天评估大鼠神经功能。末次治疗后,采用硫代巴比妥酸(TBA)方法、黄嘌呤氧化酶法、比色法分别对应检测血清丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)等氧化应激指标水平,采用酶联免疫吸附法(ELISA)检测血清肿瘤坏死因子(TNF)-α、白细胞介素(IL)-1β等炎症指标水平,采用末端脱氧核苷酸转移酶介导的d UTP缺口末端标记(TUNEL)法检测脑组织神经细胞凋亡水平,采用蛋白免疫印迹法检测脑组织血管内皮细胞生长因子(VEGF)、碱性成纤维细胞生长因子(bFGF)蛋白表达水平,采用实时定量聚合酶链反应(q RT-PCR)法检测脑组织VEGF、b FGF mRAN表达水平。【结果】干预第1、7、14天:与假手术组比较,模型组神经功能缺损评分升高(P<0.05);与模型组比较,电针组、中药组、针药组神经功能缺损评分降低(P<0.05)。末次治疗后:与假手术组比较,模型组大鼠血清MDA、TNF-α及IL-1β水平升高,SOD、GSH-Px水平降低,神经细胞凋亡率升高,VEGF、bFGF的蛋白、m RNA表达水平降低(均P<0.05);与模型组比较,电针组、中药组、针药组大鼠血清MDA、TNF-α及IL-lβ水平降低,SOD、GSH-Px水平降低,神经细胞凋亡率降低,VEGF、bFGF的蛋白、mRNA表达水平升高(均P<0.05)。针药组的作用效果均优于电针组、中药组(P<0.05)。【结论】电针联合大黄、水牛角煎液可通过降低氧化应
Objective To observe the therapeutic mechanism of electro-acupuncture combined with decoction of Radix et Rhizoma Rhei and Cornu Bubali for rats with cerebral infarction.Methods Forty rats were randomly divided into five groups,namely,sham-operation group,model group,electro-acupuncture group,Chinese herbal medicine group and acupuncture-medicine group,with 8 rats in each group.A model of cerebral infarction was established in all rats except the sham-operation group,while the sham-operation group was given no ligation of the arteries after isolation.After successful modeling,the electro-acupuncture group was treated with acupuncture at Baihui(DU20),Zusanli(ST36)and Shuigou(DU26)points,the Chinese herbal medicine group was treated with 5 g/kg of decoction of Radix et Rhizoma Rhei and Cornu Bubali by gavage,the acupuncture-medicine group was given the same intervention therapies as the electro-acupuncture group and the Chinese herbal medicine group,and the model group and the sham-operation group were treated with an equal volume of normal saline by gavage,the treatment lasting for 14 days.The neurological functions of the rats were assessed on day 1,7 and14 of the intervention.After the final treatment,the serum levels of oxidative stress indicators such as malondialdehyde(MDA),superoxide dismutase(SOD)and glutathione peroxide(GSH-Px)were measured using the thiobarbituric acid(TBA)method,xanthine oxidase method and colorimetric method correspondingly.The serum levels of tumour necrosis factor(TNF)-α,interleukin(IL)-1βinflammatory indicators were measured by enzyme-linked immunosorbent assay(ELISA).A terminal deoxynucleotidyl transferase-mediated d UTP nick end labeling(TUNEL)assay was used to detect apoptotic levels of neuronal cells in brain tissues.The protein expression levels of vascular endothelial growth factor(VEGF)and basic fibroblast growth factor(b FGF)in brain tissues were detected by protein immunoblotting,and the mRAN expression levels of VEGF and bFGF in brain tissues were detected by real-time qu
作者
赵辉
杨晓静
程芳
沈菲
张静
高宏波
袁玉欣
ZHAO Hui;YANG Xiao-Jing;CHENG Fang;SHEN Fei;ZHANG Jing;GAO Hong-Bo;YUAN Yu-Xin(Dept.of Rehabilitation Medicine,Hebei Veterans General Hospital,Xingtai 054000 Hebei,China)
出处
《广州中医药大学学报》
CAS
2022年第5期1116-1122,共7页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(编号:81473268)
邢台市科技计划项目(编号:2017ZC057)
关键词
电针
大黄
水牛角
脑梗死
氧化应激
炎症反应
血管生成
大鼠
electro-acupuncture
Radix et Rhizoma Rhei
Cornu Bubali
cerebral infarction
oxidativestress
inflammatory response
angiogenesis
rats
