摘要
目的研究胃癌患者外周血记忆性干细胞样T细胞(memory stem cell T cells,TSCM)的分布特征,分析TSCM与临床病理特征的关系,探讨其临床应用价值。方法选取2018年7—12月在我院住院的胃癌患者32例为病例组,同期健康志愿者23例作为对照组。流式细胞术检测胃癌患者外周血TSCM、初始T细胞(naive T cells,TN)、记忆性T细胞(memory T cells,TM)的比例,并比较其与对照组的差异,分析TN、TSCM、TSCM/TN值、TM与临床病理特征的关系。结果病例组患者外周血TN比例、TSCM比例均明显低于健康对照组,TSCM/TN值明显高于健康对照组,差异均有统计学意义(P<0.05)。TN比例与肿瘤TNM分期、浸润深度呈负相关,与组织分化程度呈正相关;TSCM/TN值与肿瘤TNM分期、浸润深度、淋巴结转移呈正相关。结论胃癌患者外周血TN、TSCM/TN值与肿瘤分期、浸润深度等临床病理特征密切相关,可作为一种新的方式用于评价患者的免疫状态;TSCM在胃癌的过继细胞免疫治疗中可能有重要的临床应用价值,但需大规模多中心临床研究进一步评价。
Objective To study the distribution characteristics of peripheral blood stem cell-like memory T cells(TSCM)in patients with gastric cancer and to analyze their relationship to clinicopathological characteristics and clinical application value. Methods Thirty-two patients hospitalized with gastric cancer in our hospital from July to December, 2018 were selected as the case group. Twenty-three healthy volunteers recruited during the same period served as the control group. The percentages of TSCM, naive T cells(TN)and memory T cells(TM)were detected by flow cytometry and the differences between the two groups were compared. The relationship of clinicopathological features to TN, TSCM, TSCM/TN ratio and TM was analyzed. Results The percentages of TN and TSCM in peripheral blood of the patients were significantly lower than those of the healthy control group, while the ratio of TSCM to TN was significantly increased in the case group(all P<0.05). The proportion of TN was negatively correlated with tumor TNM stage and invasion depth, and positively correlated with the degree of tissue differentiation. The ratio of TSCM to TN was positively correlated with TNM stage, invasion depth and lymph node metastasis. Conclusions TN and the ratio of TSCM to TN in peripheral blood of patients with gastric cancer are closely related to clinical and pathological characteristics such as tumor stage and invasion depth, which could be used as a new way to evaluate the immune status of patients. TSCM might have important clinical application value in adoptive cellular immunotherapy of gastric cancer, but large-scale multi-center clinical studies are needed for further evaluation.
作者
王子文
李莉
刘珺良
李慧
谭宁平
朱祖安
Wang Ziwen;Li Li;Liu Junliang;Li Hui;Tan Ningping;Zhu Zu′an(Clinical College of Xuzhou Medical University, Xuzhou 221000, China;Department of Gastroenterology, the Affiliated Hospital of Xuzhou Medical University, Xuzhou 221000, China)
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2019年第6期448-453,共6页
Chinese Journal of Microbiology and Immunology
基金
江苏省卫生计生委科研项目(H2017082)
徐州市重点研发计划项目(KC17166).
