摘要
目的探讨外周血干细胞样记忆性T细胞(Tscm)和调节性T细胞(Treg)在卵巢癌患者外周血中的表达及临床意义。方法选取行腹腔镜切除根治术治疗的卵巢癌患者83例作为研究组,以60例同期体检的健康志愿者作为对照组,采集研究组术后24 h内、对照组体检当日的外周血,检测Tscm及Treg比率,分析卵巢癌患者外周血Tscm、Treg比率与临床病理特征的关系;术后随访3年,统计卵巢癌患者生存情况,并比较死亡组和生存组患者的Tscm、Treg比率;采用Cox回归分析影响卵巢癌患者预后的因素,绘制受试者工作特征曲线(ROC),以曲线下面积(AUC)评价外周血Tscm、Treg对卵巢癌患者预后的预测效能。结果研究组Tscm比率明显低于对照组、Treg比率明显高于对照组,差异有统计学意义(P<0.001);不同肿瘤大小、临床分期、分化程度、有无淋巴结转移、有无腹水卵巢癌患者外周血Tscm、Treg比率比较,差异有统计学意义(P<0.05);死亡组患者Tscm比率低于生存组、Treg比率高于生存组,差异有统计学意义(P<0.05);Cox回归分析显示临床分期、分化程度、淋巴结转移、外周血Tscm、Treg比率均为影响卵巢癌患者预后的独立影响因素(P<0.05);ROC分析显示,外周血Tscm、Treg比率单独及两者联合预测卵巢癌患者预后的灵敏度分别为82.61%、69.57%、69.57%,特异度分别为70.69%、75.86%、92.28%,AUC分别为0.756、0.743、0.892,。结论卵巢癌患者外周血Tscm比率偏低、Treg比率偏高,两者联合预测卵巢癌患者预后的效能较高。
Objective To investigate the expression and clinical significance of stem cell-like memory T cells(Tscm)and regulatory T cells(Treg)in the peripheral blood of patients with ovarian cancer.Methods A total of 83 patients with ovarian cancer undergoing laparoscopic radical resection were selected as study group,and 60 healthy volunteers undergoing physical examination at the same time were selected as control group.Peripheral blood was collected from study group within 24 hours after operation and control group on the day of physical examination.Tscm and Treg were measured.Tscm/Treg was calculated.The association of Tscm,Treg and Tscm/Treg in peripheral blood with clinicopathological characteristics of the patients with ovarian cancer were analyzed.The patients were followed up for 3 years,and the survival of the patients with ovarian cancer was documented.The ratios of Tscm to Treg were compared between dead and alive groups.Cox regression analysis was used to analyze the factors affecting the prognosis of the patients with ovarian cancer.Receiver operating characteristic curve(ROC)was plotted.The area under the ROC curve(AUC)was used to evaluate the predictive efficacy of peripheral blood Tscm and Treg in the prognosis of the patients with ovarian cancer.Results Tscm percentage in study group was significantly lower than that in control group,while Treg percentage was significantly higher in study group than that in control group(P<0.001).The percentages of peripheral blood Tscm and Treg were significantly associated with tumor size,clinical stage,differentiation degree,whether there was lymph node metastasis and whether there was ascites(P<0.05).The percentage of Tscm in dead group was lower than that in alive group,while Treg was higher in dead group than that in the alive group(P<0.05).Cox regression analysis showed that clinical stage,differentiation degree,lymph node metastasis,the percentages of peripheral blood Tscm and Treg were independent factors affecting the prognosis of the patients with ovarian cance
作者
袁帅
朱元媛
周雅娟
龚咪
YUAN Shuai;ZHU Yuanyuan;ZHOU Yajuan;GONG Mi(Department of Gynecology,Huaian First Hospital Affiliated to Nanjing Medical University,Huaian 223300,Jiangsu,China)
出处
《贵州医科大学学报》
CAS
2022年第12期1434-1439,共6页
Journal of Guizhou Medical University
基金
江苏省卫生计生委科研课题(BJ17014)。
关键词
干细胞样记忆性T细胞
调节性T细胞
卵巢癌
临床病理特征
stem cell like memory T cells(Tscm)
regulatory T cells(Treg)
ovarian cancer
clinicopathological characteristics
