摘要
利用生物信息学方法,全面分析了桑树NBS-LRR类基因家族组成、结构、进化、组织表达,并对该家族基因的调控microRNA(miRNA)进行了预测。共筛选到112个桑树NBS-LRR类基因,根据功能域主要分为NBS、CC-NBS、CC-NBS-LRR、NBS-LRR 4种类型。内含子数和相位分析结果显示,基因结构类型多样。聚类分析结果显示不同聚类组间存在较多的类型交叉现象。该家族基因存在组织表达特异性。大部分桑树NBS-LRR类基因均具有被miRNAs调控的可能性,miR472b和miR482b在调控该家族基因表达中可能发挥了主要作用。桑树NBS-LRR家族基因结构和进化的复杂性决定了其功能的多样性,miRNA在控制该家族基因表达的适应性成本中发挥作用。
The composition,structure,evolution,tissue expression of the nucleotide-binding site(NBS)leucine-rich repeat(LRR)gene family in mulberry,and the relationship between microRNA(miRNA)and the family gene were analyzed by bioinformatics.A total of 112 NBS-LRR genes of mulberry were identified,which were divided into four types(NBS,CC-NBS,CC-NBS-LRR and NBS-LRR)according to functional domains.The results of intron number and phase analysis showed that the types of genetic structure were various.Cluster analysis results showed that many types gene were mixed.The family genes were specific in tissue expression.Most of the NBS-LRR genes in mulberry were potential targets of miRNAs,and miR472b and miR482b played a major role in regulating the gene expression of the family.The complexity of the genetic structure and evolution of the NBS-LRR family of mulberry determines the diversity of its function.miRNA may play a role in reducing the adaptive cost of gene expression.
作者
刘潮
褚洪龙
韩利红
杨云锦
高永
唐利洲
LIU Chao;CHU Hong-long;HAN Li-hong;YANG Yun-jin;GAO Yong;TANG Li-zhou(Center for Yunnan Plateau Biological Resources Protection and Utilization/College of Biological Resource and Food Engineering/Key Laboratory of Yunnan Province Universities of the Diversity and Ecological Adaptive Evolution for Animals and Plants on Yungui Plateau,Qujing Normal University,Qujing 655011,China)
出处
《江苏农业学报》
CSCD
北大核心
2019年第3期544-553,共10页
Jiangsu Journal of Agricultural Sciences
基金
国家自然科学基金项目(31460561、31760013、31860005、31860057)
云南省地方本科高校基础研究联合专项(2017FH001-034、2017FH001-037)
国家级大学生创新创业训练计划项目(201610684004、201710684012)
关键词
桑树
NBS-LRR
生物信息学
MIRNA
mulberry
nucleotide-binding site leucine-rich repeat(NBS-LRR)
bioinformatics
miRNA
