摘要
为研究云南野生蔷薇属中的NBS类抗病基因,根据已知抗病基因NBS-LRR序列中的保守区域设计简并引物,利用RT-PCR技术从云南悬钩子蔷薇中进行体外扩增,获得了对应区域的cDNA片段,回收、克隆这些特异片段,测序分析,共得到4个含有NBS-LRR保守结构域的抗病基因同源序列(RGAs),分别命名为AC9、AC39、AC50和AC68。它们与已报道的11个NBS类抗病基因相应区段的氨基酸序列相似性为5.4%～79.2%,其中这4个RGAs片段与Mi、RPS2、Pib和RPM1基因聚为一类。表明这4条RGAs序列可进一步用作悬钩子蔷薇抗病候选基因的分子筛选及遗传图谱的构建。
In the study of NBS resistance genes of Yunnan wild Rosa, the degenerate oligonucleotide primers were designed according to the conserved motifs NBSLRR of most plant resistance （R） genes. The cDNA fragments were isolated from Rosa rubus by reverse transcription PCR （RTPCR）. Then four resistance gene analogs （RGAs） were sequenced and named as AC9, AC39, AC50 and AC68, which contain NBSLRR domain. The sequence of each RGAs was compared with NBS region of eleven resistance genes reported before, and the similarities of aminoacid sequences ranged from 5.4% to 79.2%, and these four RGAs were clustered a group with Mi、RPS2、Pib and RPM1 gene. These results showed that the four RGAs will be used as molecular markers for screening candidate disease resistance genes and genetic map construction of Rosa rubus in the future.
出处
《植物分类与资源学报》
CAS
CSCD
北大核心
2012年第1期56-62,共7页
Plant Diversity
基金
国家自然科学基金项目(31160403)
云南省应用基础研究计划项目(2011FB124)
科技部863项目(2011AA100208)
云南省攻关项目(2011BB013)
农业部948项目(2011-G17)
关键词
悬钩子蔷薇
保守区域
抗病基因同源序列
简并引物
Rosa rubus Conserved motif Resistance gene analogs （RGAs） Degenerate oligonucleotide primers
