摘要
为研究microRNA(miRNA)在五指山猪和长白猪肌肉生长发育中的差异和探究差异miRNA对骨骼肌发育转录后调控的影响,以1月龄的五指山猪和长白猪背最长肌为材料,通过转录组测序和生物信息学分析筛选与骨骼肌发育相关的差异miRNA。结果显示,从2个文库中共鉴定获得318种己知的猪miRNA,五指山猪和长白猪分别鉴定出312个和306个已知miRNA,同时分别发现了83个和88个新的miRNA。获得17个差异显著的miRNA,其中16个miRNA表达上调,1个miRNA表达下调。靶基因预测和生物学功能预测发现17个差异miRNA,共预测到535个靶基因,靶向作用于mTOR信号通路和肌动蛋白细胞骨架调控的ssc-miR-486,ssc-miR-204,ssc-miR-338和ssc-miR-885-3p 4个miRNA可能参与骨骼肌生长发育过程。
To investigate the differences of microRNA(miRNA)in growth and development of skeletal muscle between Wuzhishan pig and Landrace and to explore the effects of differential miRNAs on post-transcriptional regulation of skeletal muscle development,the longissimus dorsi muscles of one-month-old Wuzhishan pig and Landrace were used as materials to screen their differential miRNAs related to skeletal muscle development by transcriptome sequencing and bioinformatics analysis.A total of 318 known porcine miRNAs were identified from two libraries,312 and 306 known miRNAs were identified in Wuzhishan and Landrace pigs respectively,and 83 and 88 new miRNAs were found at the same time.Seventeen differential miRNAs were obtained,of which 16 miRNAs were up-regulated and one miRNA was down-regulated.A total of 535 target genes were predicted by 17 differential miRNAs,targeting ssc-miR-486,ssc-miR-204,ssc-miR-338 and ssc-miR-885-3p,which were regulated by the mTOR signaling pathway and actin cytoskeleton.The four miRNAs can participate in the growth and development of skeletal muscle.
作者
孙瑞萍
王峰
晁哲
刘海隆
郑心力
刘圈炜
黄丽丽
邢漫萍
魏立民
SUN Rui-ping;WANG Feng;CHAO Zhe;LIU Hai-long;ZHENG Xin-li;LIU Quan-wei;HUANG Li-li;XING Man-ping;WEI Li-min(Institute of Animal Science and Veterinary Medicine,Hainan Academy of Agricultural Science,Haikou 571100,China;Hainan Key Lab of Tropical Animal Reproduction&Breeding and Epidemic Disease Research,Haikou 571100,China)
出处
《江苏农业学报》
CSCD
北大核心
2020年第3期620-625,共6页
Jiangsu Journal of Agricultural Sciences
基金
海南省农业科学院农业科技创新专项项目(cxzx201607)
国家自然科学基金项目(31560628,31760646)
海南省省属科研院所技术开发研究专项(KYYS-2015-02)
中央引导地方科技发展专项资金项目(ZY2019HN01)。
关键词
五指山猪
骨骼肌
miRNA高通量测序
Wuzhishan pig
skeletal muscle
microRNA
high-throughput sequencing
