摘要
为研究microRNA(miRNA)在五指山猪和长白猪肌肉发育过程中的差异及其对骨骼肌发育转录后调控的影响,以8月龄的五指山猪和长白猪背最长肌为材料,通过Solexa测序技术结合生物信息学筛选与骨骼肌发育相关的差异miRNAs。结果从2个文库中共鉴定获得311种己知的猪miRNAs,五指山猪和长白猪分别鉴定出300和293个已知miRNAs,同时分别发现了96和79新的miRNAs。筛选到的17个差异的miRNAs中有15个miRNAs在五指山猪背肌中表达量显著下调,2个miRNAs表达量显著上调。这些差异miRNAs靶基因预测共靶向574个基因,富集到30个差异显著生物学通路(P<0.05),其中富集到胰岛素信号通路的miRNAs有5个(ssc-miR-362,ssc-miR-455-3p,ssc-miR-497,ssc-miR-499-5p和ssc-miR-874)。本研究从miRNA层面揭示了8月龄五指山猪和长白猪骨骼肌生长发育的差异,为进一步研究骨骼肌生长发育的分子机理提供基础数据。
In order to investigate the differences of microRNAs(miRNAs)in growth and development of skeletal muscle between Wuzhishan and Landrace pigs and to explore the effects of differential miRNAs on post-transcriptional regulation of skeletal muscle development,we used the longissimus dorsi muscle of 8-month-old Wuzhishan pig and of Landrace pig to screen their differential miRNAs related to the development of skeletal muscle via Solexa sequencing combined with bioinformatics analysis.A total of 311 known porcine miRNAs were identified from 2 libraries,300 and 293 known miRNAs were identified in Wuzhishan and Landrace pigs respectively,and new identified miRNAs were 96 and 79 respectively.Fifteen of 17 screened differential miRNAs significantly down-regulated in the Landrace pig,while 2 miRNAs significantly up-regulated.A total of 574 target genes were predicted by the 17 differential miRNAs that enriched to 30 significantly different biological pathways(P<0.05),of which 5 miRNAs enriched to the insulin signaling pathway(ssc-miR-362,ssc-miR-455-3p,ssc-miR-497,ssc-miR-499-5p,and ssc-miR-874).This study reveals the differences in the growth and development of skeletal muscle between 8-month-old Wuzhishan pig and Landrace pig at the level of miRNA and provides basic data for further research on the molecular mechanism of skeletal muscle growth.
作者
孙瑞萍
王峰
晁哲
刘海隆
邢漫萍
刘圈炜
郑心力
黄丽丽
魏立民
SUN Rui-ping;WANG Feng;CHAO Zhe;LIU Hai-long;XING Man-pin;LIU Quan-wei;ZHENG Xin-li;HUANG Li-li;WEI Li-min(Institute of Animal Science and Veterinary Medicine,Hainan Academy of Agricultural Science,Haikou 571100;Hainan Key Lab of Tropical Animal Reproduction&Breeding and Epidemic Disease Research,Haikou 571100)
出处
《生物技术通报》
CAS
CSCD
北大核心
2020年第10期40-48,共9页
Biotechnology Bulletin
基金
海南省农业科学院农业科技创新专项项目(cxzx201607)
国家自然科学基金项目(31760646)
海南省省属科研院所技术开发研究专项(KYYS-2015-02)
中央引导地方科技发展专项资金(ZY2019HN01)。
