摘要
试验用实时定量PCR方法研究了蓝塘猪和长白猪1、27、90、150和180日龄肝脏和肌肉组织中IGF-Ⅰ、IGF-ⅠR和IGFBP3基因表达的发育性变化。结果显示:(1)肝脏中IGF-Ⅰ基因表达量高于肌肉组织。两个品种肌肉中表达量均是90日龄时最高;肝脏中则出现品种差异,长白猪180日龄时最高,而蓝塘猪90日龄时最高。(2)肝脏中IGFBP3基因表达量高于肌肉组织。两个品种肌肉中IGFBP3基因表达量均是1日龄最高,蓝塘猪1日龄与其它各阶段差异显著(P<0.05),长白猪1日龄与90、150和180日龄差异显著(P<0.05);肝脏中表达规律出现品种差异,蓝塘猪90日龄时肝脏中表达量最高,且与1、27和150日龄差异显著(P<0.05);长白猪150日龄最高,各阶段差异不显著。(3)各阶段肝脏中IGF-ⅠR基因表达量变化较大,蓝塘猪90日龄时最高,长白猪27日龄时最高。肌肉中表达规律基本一致,均是出生时最高,随年龄增加有下降趋势。(4)3个基因比较,蓝塘猪肝脏中IGF-Ⅰ、IGF-ⅠR和IGFBP3基因和肌肉中IGF-Ⅰ基因均是90日龄时表达量最高,肌肉中IGF-ⅠR和IGFBP3基因1日龄时表达量最高;长白猪肌肉中3个基因表达规律相似,表现出生长前期表达量较高,后期下降的趋势,而肝脏中变化规律不明显。
Real time RT-PCR was applied to compare the developmental expression patterns of IGF-Ⅰ, IGF-ⅠR and IGFBP3 genes in liver and muscle of Lantang and Landrace pigs at the ages of 1, 27, 90, 150 and 180 days. We have found： （1） IGF-Ⅰ gene was expressed at a higher level in liver than that in muscle. The highest level of IGF-Ⅰ mRNA was detected in muscle at day 90 compared with other ages in both breeds; however, the level of IGF-Ⅰ mRNA differed between breeds and the highest level was observed in liver at day 180 in Landrace and at day 90 in Lantang . （2） The expression level of IGFBP3 gene was higher in liver than that in muscle, the highest expression level of IGFBP3 was observed in muscle, at day 1 in both breeds. Significantly higher level of IGFBP3 mRNA at day 1 in Lantang were detected （P 〈 0.05） ; for Landrace, a significantly higher level was detected at clay 1 than that at the clays of 90, 150 and 180 （P〈0.05）. However, expression of IGFBP3 differed between breeds in liver. Significantly higher expression level of IGFBP3 was observed in Lantang at day 90 than that at the days of 1, 27 and 150 （P 〈0.05）, whereas the highest level was found in Landrace pigs at day 150 among all ages, but the difference was not statistically significant. （3） In liver, the highest expression level of IGF-ⅠR was detected at day 27 in Landrace and at day 90 in Lantang. In muscle, expression level of IGF-ⅠR was higher at birth than any other ages and decreased with age in both breeds. （4） In total, Lantang showed highest expression levels of IGF-Ⅰ, IGF-ⅠR and IGFBP3 genes in liver, and IGF-Ⅰ gene in muscle at day 90, compared with other ages; while the highest levels of IGF-ⅠR and IGFBP3 genes in muscle were observed at day 1. Generally, the expression pattern of all three genes seemed to be similar in muscle of Landrace, with higher level at early age than that at later stage. There was no consistent varying expression pattern of all three genes in liver of Landrace.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2008年第7期866-872,共7页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金(30571327)
广东省科技计划农业攻关项目(2006B20201051
2007B020706004)资助
