摘要
目的：建立HPLC-DAD法同时测定石上柏中穗花杉双黄酮、扁柏双黄酮、银杏双黄酮的含量。方法：色谱柱：DikmaDiamons C18柱（150 mm×4.6 mm,5μm）;流动相：乙腈-0.1%甲酸水,梯度洗脱;检测波长：330 nm;柱温：25℃;流速：0.6 mL/min;进样量：10μL。结果：3种双黄酮在上述色谱条件下分离度良好,穗花杉双黄酮的线性范围为0.68～4.08 mg/mL,标准曲线为Y=5293X-2881.8（R2=0.9995）;扁柏双黄酮的线性范围为0.45～2.69 mg/mL,标准曲线为Y=1052.1X-242.58（R2=0.9996）;银杏双黄酮的线性范围为0.70～4.13 mg/mL,标准曲线为Y=1513X-1441.6（R2=0.9995）。方法学考察,精密度、稳定性RSD〈2.0%,穗花杉双黄酮、扁柏双黄酮和银杏双黄酮的平均加样回收率分别为99.03%、97.65%、93.16%,（RSD〈3.0%）。10个产地石上柏中穗花杉双黄酮的含量在0.232%～0.309%,扁柏双黄酮的含量在0.040%～0.137%,银杏双黄酮的含量在0.043%～0.074%。结论：不同产地石上柏的3种双黄酮化合物的含量有一定的差异,可能与不同地域,不同采收时间以及不同的加工方法有关。该方法操作简便,测定准确,可用于中药石上柏的质量分析。
Objective：To establish a HPLC-DAD method for the simultaneous determination of amentoflavone,hinokiflavone,ginkgetin in Selaginella doederleinii. Methods：The chromatographic separation was performed on a DikmaDiamons C18 column（150 mm×4.6 mm,5 μm）,adopting acetonitrile-0.1% formic acid solution as the mobile phase,and the detection wavelength at 330 nm,the column temperature was set at 25 ℃ and the flow rate was 0.6 mL/min,the injection volume was 10 μL. Results：This method had good linear relationship in range of 3.1-15.6 mg/mL and standard curve line of Y=5293 X-2881.8（R2=0.9995）for amentoflavone,1.1-5.6 mg/mL and standard curve line of Y=1052.1 X-242.58（R2=0.9996）for hinokiflavone and 0.7-3.4 mg/mL and standard curve line of Y=1513 X-1441.6（R2=0.9995）for ginkgetin,the RSD of precision and repeatability were less than 2.0%,the average recoveries（n=6）of amentoflavone,hinokiflavone and ginkgetin were 99.03%,97.65%,93.16%（RSD〈3.0%）. The content range of amentoflavone was 0.232%-0.309%,the content range of hinokiflavone was 0.040%-0.137%,and the content range of ginkgo was 0.043%-0.074%. Conclusion：There are some differences on the content of three bioflavonoids from different habitats,which may be related to different regions,different harvest times and different processing methods.
作者
蒋永梅
田雨佳
钱艳
黎丹
王刚
JIANG Yongmei;TIAN Yujia;QIAN Yan;LI Dan;WANG Gang(Zunyi Medical University,Zunyi 563003,Guizhou,China)
出处
《辽宁中医药大学学报》
CAS
2018年第11期34-37,共4页
Journal of Liaoning University of Traditional Chinese Medicine
基金
国家自然科学基金项目(81560569)
贵州省中医药管理局中医药、民族医药科学技术研究课题(D-449)
遵义医学院博士启动基金项目(F-742)
